H Meyer-Lueckel1, R J Wierichs2, B Gninka3, P Heldmann3, C E Dörfer3, S Paris4. 1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany. 2. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany. Electronic address: rwierichs@ukaachen.de. 3. Clinic for Operative Dentistry and Periodontology, School of Dental Medicine, Christian-Albrechts-Universitätzu Kiel, Arnold-Heller-Str. 3, 24105, Kiel, Germany. 4. Department of Operative and Preventive Dentistry, Charité-Universitätsmedizin Berlin, Assmannshauser Str. 4-6, 14197 Berlin.
Abstract
OBJECTIVES: The aim of this exploratory double-blinded, randomized, cross-over, in situ study was to compare the effects of various model parameters ('intervention', 'brushing', 'position') on enamel caries lesions in a dose-response model. METHODS: In each of four experimental legs of four weeks 16 participants wore intraoral mandibular appliances with four 'plaque-retaining' and four 'easily cleanable' positioned pre-demineralized bovine enamel specimens in the vestibular flanges mimicking proximal and buccal surfaces, respectively (n=512). The four randomly allocated interventions (either application only or brushing) included the following dentifrices: AlF3 1360ppmF(-)+chlorhexidine 0.05% (Lacalut aktiv, LA1360), NaF 1,450ppmF(-)(Blend-a-Med ProExpert), NaF 500ppm F(-) and 0ppm F(-) as negative control (NC) (both experimental, based on Blend-a-Med ProExpert). RESULTS: Differences in integrated mineral loss (ΔΔZ) and lesion depth (ΔLD) were calculated between values before and after the in situ period using transversal microradiography. Significant differences for ΔΔZ [adjusted mean (95% CI))] were found between NC, NaF500 and LA1360for both 'plaque-retaining' [-1830 (-2371;1289); -986 (-1530;442); -2 (-548;544)vol%×μm] as well as 'easily cleanable' specimens [-399 (-682; -116); -391 (-672; -110); -16 (-302;270)vol%×μm]. Values for NaF1450 revealed a similar dose-response as LA1360.Values for LA1360 and NaF1450 did not differ significantly (p>0.05; ANCOVA). CONCLUSION/CLINICAL SIGNIFICANCE: The design of the present in situ study was able to reveal a fluoride dose-response to hamper further demineralization of enamel specimens for 'easily cleanable' and 'plaque-retaining' sites being brushed or not. Particularly 'plaque-retaining' sites seem to be recommendable for measuring potential anticaries efficacy in situ.
RCT Entities:
OBJECTIVES: The aim of this exploratory double-blinded, randomized, cross-over, in situ study was to compare the effects of various model parameters ('intervention', 'brushing', 'position') on enamel caries lesions in a dose-response model. METHODS: In each of four experimental legs of four weeks 16 participants wore intraoral mandibular appliances with four 'plaque-retaining' and four 'easily cleanable' positioned pre-demineralized bovine enamel specimens in the vestibular flanges mimicking proximal and buccal surfaces, respectively (n=512). The four randomly allocated interventions (either application only or brushing) included the following dentifrices: AlF3 1360ppmF(-)+chlorhexidine 0.05% (Lacalut aktiv, LA1360), NaF 1,450ppmF(-)(Blend-a-Med ProExpert), NaF 500ppm F(-) and 0ppm F(-) as negative control (NC) (both experimental, based on Blend-a-Med ProExpert). RESULTS: Differences in integrated mineral loss (ΔΔZ) and lesion depth (ΔLD) were calculated between values before and after the in situ period using transversal microradiography. Significant differences for ΔΔZ [adjusted mean (95% CI))] were found between NC, NaF500 and LA1360for both 'plaque-retaining' [-1830 (-2371;1289); -986 (-1530;442); -2 (-548;544)vol%×μm] as well as 'easily cleanable' specimens [-399 (-682; -116); -391 (-672; -110); -16 (-302;270)vol%×μm]. Values for NaF1450 revealed a similar dose-response as LA1360.Values for LA1360 and NaF1450 did not differ significantly (p>0.05; ANCOVA). CONCLUSION/CLINICAL SIGNIFICANCE: The design of the present in situ study was able to reveal a fluoride dose-response to hamper further demineralization of enamel specimens for 'easily cleanable' and 'plaque-retaining' sites being brushed or not. Particularly 'plaque-retaining' sites seem to be recommendable for measuring potential anticaries efficacy in situ.
Authors: Richard Johannes Wierichs; Judith Mester; Thomas Gerhard Wolf; Hendrik Meyer-Lueckel; Marcella Esteves-Oliveira Journal: Clin Oral Investig Date: 2021-11-24 Impact factor: 3.573
Authors: M Esteves-Oliveira; N M Santos; H Meyer-Lueckel; R J Wierichs; J A Rodrigues Journal: Clin Oral Investig Date: 2016-03-19 Impact factor: 3.573