M Esteves-Oliveira1, N M Santos2, H Meyer-Lueckel3, R J Wierichs3, J A Rodrigues2. 1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany. mestevesoliveira@ukaachen.de. 2. Department of Pediatric Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. 3. Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany.
Abstract
OBJECTIVES: The aim of the study was to investigate the caries-preventive effect of newly developed fluoride and fluoride-free toothpastes specially designed for erosion prevention. The hypothesis was that these products might also show superior caries-inhibiting effect than regular fluoride toothpastes, since they were designed for stronger erosive acid challenges. MATERIALS AND METHODS: Enamel specimens were obtained from bovine teeth and pre-demineralized (pH = 4.95/21 days) to create artificial caries lesions. Baseline mineral loss (ΔZB) and lesion depth (LDB) were determined using transversal microradiography (TMR). Ninety specimens with a median ΔZB (SD) of 6027 ± 1546 vol% × μm were selected and randomly allocated to five groups (n = 18). Treatments during pH-cycling (14 days, 4 × 60 min demineralization/day) were brushing 2×/day with AmF (1400 ppm F-, anti-caries [AC]); AmF/NaF/SnCl2/Chitosan (700 ppm F-/700 ppm F-/3500 ppm Sn2+, anti-erosion [AE1]); NaF/KNO3 (1400 ppm F-, anti-erosion [AE2]); nano-hydroxyapatite-containing (0 ppm F-, [nHA]); and fluoride-free toothpastes (0 ppm F-, negative control [NC]). Toothpaste slurries were prepared with mineral salt solution (1:3 wt/wt). After pH-cycling specimens presenting lesion, surface loss (mainly by NC and nHA) were discarded. For the remaining 77 specimens, new TMR analyses (ΔZE/LDE) were performed. Changes in mineral loss (ΔΔZ = ΔZB - ΔZE) and lesion depth (ΔLD = LDB - LDE) were calculated. RESULTS: All toothpastes caused significantly less demineralization (lower ΔΔZ) than NC (p < 0.05, ANOVA) except for nHA. The fluoride toothpastes did not differ significantly regarding ΔΔZ and ΔLD (p > 0.05, ANOVA). CONCLUSION/CLINICAL RELEVANCE: While both anti-erosive and anti-caries toothpastes reduced mineral loss to a similar extent, the fluoride-free nano-hydroxyapatite-containing toothpaste seemed not to be suitable for inhibition of caries demineralization in vitro.
OBJECTIVES: The aim of the study was to investigate the caries-preventive effect of newly developed fluoride and fluoride-free toothpastes specially designed for erosion prevention. The hypothesis was that these products might also show superior caries-inhibiting effect than regular fluoride toothpastes, since they were designed for stronger erosive acid challenges. MATERIALS AND METHODS: Enamel specimens were obtained from bovine teeth and pre-demineralized (pH = 4.95/21 days) to create artificial caries lesions. Baseline mineral loss (ΔZB) and lesion depth (LDB) were determined using transversal microradiography (TMR). Ninety specimens with a median ΔZB (SD) of 6027 ± 1546 vol% × μm were selected and randomly allocated to five groups (n = 18). Treatments during pH-cycling (14 days, 4 × 60 min demineralization/day) were brushing 2×/day with AmF (1400 ppm F-, anti-caries [AC]); AmF/NaF/SnCl2/Chitosan (700 ppm F-/700 ppm F-/3500 ppm Sn2+, anti-erosion [AE1]); NaF/KNO3 (1400 ppm F-, anti-erosion [AE2]); nano-hydroxyapatite-containing (0 ppm F-, [nHA]); and fluoride-free toothpastes (0 ppm F-, negative control [NC]). Toothpaste slurries were prepared with mineral salt solution (1:3 wt/wt). After pH-cycling specimens presenting lesion, surface loss (mainly by NC and nHA) were discarded. For the remaining 77 specimens, new TMR analyses (ΔZE/LDE) were performed. Changes in mineral loss (ΔΔZ = ΔZB - ΔZE) and lesion depth (ΔLD = LDB - LDE) were calculated. RESULTS: All toothpastes caused significantly less demineralization (lower ΔΔZ) than NC (p < 0.05, ANOVA) except for nHA. The fluoride toothpastes did not differ significantly regarding ΔΔZ and ΔLD (p > 0.05, ANOVA). CONCLUSION/CLINICAL RELEVANCE: While both anti-erosive and anti-caries toothpastes reduced mineral loss to a similar extent, the fluoride-free nano-hydroxyapatite-containing toothpaste seemed not to be suitable for inhibition of caries demineralization in vitro.
Authors: A T Hara; S A Kelly; C González-Cabezas; G J Eckert; A P Barlow; S C Mason; D T Zero Journal: Caries Res Date: 2009-02-10 Impact factor: 4.056
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