Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded DNA containing specific gene sequences.

Literature DB >> 198749

Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded DNA containing specific gene sequences.

Abstract

A method of enriching for long double-stranded segments of eukaryotic DNA carrying particular genes is described. A purified RNA coded for by the gene is covalently attached to biotin via the protein, cytochrome c. This modified RNA is hybridized to total nuclear, double-stranded DNA under conditions that allow the formation of R-loops. Avidin, which has a high affinity for biotin, is covalently attached to polymer spheres. The complexes of avidin-spheres with DNA:RNA-biotin R-loop hybrids band in CsCl at a much lower bouyant density than does free DNA. This density is a function of the length of DNA coupled per avidin-sphere. This method was used to prepare very long double-strands of DNA highly enriched in the coding sequences for the large rRNAs of D. melanogaster and L. donovani and the histone mRNAs of S. purpuratus.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1977 PMID： 198749 PMCID： PMC342627 DOI： 10.1093/nar/4.9.2961

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

1. The organization of sea urchin histone genes.

Authors: L H Kedes; R H Cohn; J C Lowry; A C Chang; S N Cohen
Journal: Cell Date: 1975-11 Impact factor: 41.582

2. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.

Authors: M Meselson; F W Stahl; J Vinograd
Journal: Proc Natl Acad Sci U S A Date: 1957-07-15 Impact factor: 11.205

3. A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes.

Authors: J Manning; M Pellegrini; N Davidson
Journal: Biochemistry Date: 1977-04-05 Impact factor: 3.162

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors: P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal: J Mol Biol Date: 1972-11-14 Impact factor: 5.469

Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded DNA containing specific gene sequences.

1. The organization of sea urchin histone genes.

2. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.

3. A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes.

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

5. Hybridization of filters with competitor DNA in the liquid phase in a standard and a micro-assay.

6. Hybridization of RNA to double-stranded DNA: formation of R-loops.

7. Sequence arrangement of the rDNA of Drosophila melanogaster.

8. Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome.

9. The structural organization of ribosomal DNA in Drosophila melanogaster.

10. R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

1. Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge.

2. Strand-specific attachment of avidin-spheres to double-stranded poliovirus RNA.

3. Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.

4. Sequence arrangement of the rRNA genes of the dipteran Sarcophaga bullata.

5. Sequence arrangement of the 16S and 26S rRNA genes in the pathogenic haemoflagellate Leishmania donovani.

6. Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.