Literature DB >> 2932475

Recombinant DNA studies on stored necropsy brain samples from patients with Huntington's chorea.

M Upadhyaya, G P Reynolds, P S Harper.   

Abstract

An analysis of deoxyribonucleic acid (DNA) in deep frozen brain samples taken from 100 patients with Huntington's chorea after death showed undegraded DNA in 44 cases. Of these, 16 were analysed with G8, a recombinant DNA probe, linked to the Huntington's chorea locus. In all cases unambiguous Southern blots were obtainable. No correlation between the yield of DNA and the principal storage factors was observed. The use of stored brain tissue obtained after death from patients with Huntington's chorea should be considered when analysis of DNA is needed for predictive studies, but DNA should preferably be isolated from the tissue before storage as degradation in these samples can occur.

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Year:  1985        PMID： 2932475      PMCID： PMC499447          DOI： 10.1136/jcp.38.10.1093

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  5 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  DNA markers for nervous system diseases.

Authors:  J F Gusella; R E Tanzi; M A Anderson; W Hobbs; K Gibbons; R Raschtchian; T C Gilliam; M R Wallace; N S Wexler; P M Conneally
Journal:  Science       Date:  1984-09-21       Impact factor: 47.728

3.  Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.

Authors:  D Page; B de Martinville; D Barker; A Wyman; R White; U Francke; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

4.  A polymorphic DNA marker genetically linked to Huntington's disease.

Authors:  J F Gusella; N S Wexler; P M Conneally; S L Naylor; M A Anderson; R E Tanzi; P C Watkins; K Ottina; M R Wallace; A Y Sakaguchi
Journal:  Nature       Date:  1983 Nov 17-23       Impact factor: 49.962

5.  Linkage analysis of two cloned DNA sequences flanking the Duchenne muscular dystrophy locus on the short arm of the human X chromosome.

Authors:  K E Davies; P L Pearson; P S Harper; J M Murray; T O'Brien; M Sarfarazi; R Williamson
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971
  5 in total
  1 in total

1.  Neuropathological diagnosis and CAG repeat expansion in Huntington's disease.

Authors:  J H Xuereb; J C MacMillan; R Snell; P Davies; P S Harper
Journal:  J Neurol Neurosurg Psychiatry       Date:  1996-01       Impact factor: 10.154
  1 in total

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