Alexandra Lykoudi1, Aggeliki Kolialexi2, George I Lambrou3, Maria Braoudaki4, Charalampos Siristatidis5, George Konstantinos Papaioanou5, Maria Tzetis6, Ariadni Mavrou6, Nikolas Papantoniou5. 1. 3rd Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens Medical School, Athens, Greece; Department of Medical Genetics, National and Kapodistrian University of Athens Medical School, Athens, Greece. 2. 3rd Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens Medical School, Athens, Greece; Department of Medical Genetics, National and Kapodistrian University of Athens Medical School, Athens, Greece. Electronic address: akolial@med.uoa.gr. 3. First Department of Pediatrics, National and Kapodistrian University of Athens, Choremeio Research Laboratory, Thivon & Levadeias, 11527, Athens, Greece. 4. Department of Medical Genetics, National and Kapodistrian University of Athens Medical School, Athens, Greece; School of Life and Medical Sciences, University of Hertfordshire, Hatfield, AL10 9AB, United Kingdom. 5. 3rd Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens Medical School, Athens, Greece. 6. Department of Medical Genetics, National and Kapodistrian University of Athens Medical School, Athens, Greece.
Abstract
INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies. METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction. RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR. DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.
INTRODUCTION: To determine the miRNA expression profile in placentas complicated by Preeclampsia (PE) and compare it to uncomplicated pregnancies. METHODS: Sixteen placentas from women with PE, [11 with early onset PE (EOPE) and 5 with late onset PE (LOPE)], as well as 8 placentas from uncomplicated pregnancies were analyzed using miRNA microarrays. For statistical analyses the MATLAB® simulation environment was applied. The over-expression of miR-518a-5p was verified using Quantitative Real-Time Polymerase Chain Reaction. RESULTS: Forty four miRNAs were found dysregulated in PE complicated placentas. Statistical analysis revealed that miR-431, miR-518a-5p and miR-124* were over-expressed in EOPE complicated placentas as compared to controls, whereas miR-544 and miR-3942 were down-regulated in EOPE. When comparing the miRNA expression profile in cases with PE and PE-growth restricted fetuses (FGR), miR-431 and miR-518a-5p were found over-expressed in pregnancies complicated by FGR. DISCUSSION: Since specific miRNAs can differentiate EOPE and LOPE from uncomplicated placentas, they may be considered as putative PE-specific biomarkers. MiR-518a-5p emerged as a potential diagnostic indicator for EOPE cases as well as for PE-FGR complicated placentas, indicating a potential link to the severity of the disease.
Authors: Katherine R Millen; Catalin S Buhimschi; Guomao Zhao; Kara M Rood; Sammy Tabbah; Irina A Buhimschi Journal: Hypertension Date: 2018-04-23 Impact factor: 10.190
Authors: Tania L Gonzalez; Laura E Eisman; Nikhil V Joshi; Amy E Flowers; Di Wu; Yizhou Wang; Chintda Santiskulvong; Jie Tang; Rae A Buttle; Erica Sauro; Ekaterina L Clark; Rosemarie DiPentino; Caroline A Jefferies; Jessica L Chan; Yayu Lin; Yazhen Zhu; Yalda Afshar; Hsian-Rong Tseng; Kent Taylor; John Williams; Margareta D Pisarska Journal: Epigenomics Date: 2021-05-25 Impact factor: 4.778