Danai Mavreli1,2, Alexandra Lykoudi1,2, George Lambrou3, George Papaioannou1, Nikolas Vrachnis1, Sophia Kalantaridou1, Nikolas Papantoniou1, Aggeliki Kolialexi4,2. 1. 3 Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Athens, Greece. 2. Department of Medical Genetics, National and Kapodistrian University of Athens, Athens, Greece. 3. 1 Department of Pediatrics, National and Kapodistrian University of Athens, Athens, Greece. 4. 3 Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Athens, Greece akolial@med.uoa.gr.
Abstract
BACKGROUND/AIM: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies. MATERIALS AND METHODS: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. RESULTS: miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. CONCLUSION: miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate non-invasive biomarkers for early prediction and prevention. Copyright
BACKGROUND/AIM: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies. MATERIALS AND METHODS: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. RESULTS:miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. CONCLUSION:miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate non-invasive biomarkers for early prediction and prevention. Copyright
Authors: Alexandra Lykoudi; Aggeliki Kolialexi; George I Lambrou; Maria Braoudaki; Charalampos Siristatidis; George Konstantinos Papaioanou; Maria Tzetis; Ariadni Mavrou; Nikolas Papantoniou Journal: Placenta Date: 2017-11-14 Impact factor: 3.481
Authors: Ryan M O'Connell; Daniel Kahn; William S J Gibson; June L Round; Rebecca L Scholz; Aadel A Chaudhuri; Melissa E Kahn; Dinesh S Rao; David Baltimore Journal: Immunity Date: 2010-09-30 Impact factor: 31.745