| Literature DB >> 34030457 |
Tania L Gonzalez1, Laura E Eisman1, Nikhil V Joshi1,2, Amy E Flowers1, Di Wu3, Yizhou Wang3, Chintda Santiskulvong4, Jie Tang5, Rae A Buttle1, Erica Sauro1, Ekaterina L Clark1, Rosemarie DiPentino1, Caroline A Jefferies6, Jessica L Chan1,2, Yayu Lin1, Yazhen Zhu7, Yalda Afshar2, Hsian-Rong Tseng7, Kent Taylor2,8, John Williams1,2, Margareta D Pisarska1,2,5.
Abstract
Aim: To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Materials & methods: Using next-generation sequencing, we characterize the normative human placenta miRNome in first (n = 113) and third trimester (n = 47). Results & conclusion: There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (p ≥ 0.05, fold change ≤2) and 180 significantly different (false discovery rate <0.05, fold change >2). Of placenta-specific miRNA clusters, chromosome 14 miRNA cluster decreases across gestation and chromosome 19 miRNA cluster is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae.Entities:
Keywords: chorionic villi; gestational differences; human transcriptome; miRNA; miRNA atlas; miRNome; placenta; pregnancy; stable miRNAs; uncomplicated pregnancies
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Year: 2021 PMID： 34030457 PMCID： PMC8244582 DOI： 10.2217/epi-2021-0055
Source DB: PubMed Journal: Epigenomics ISSN： 1750-192X Impact factor: 4.778