| Literature DB >> 29248418 |
Tomokazu Kuchibiro1, Masaru Komatsu2, Katsutoshi Yamasaki3, Tatsuya Nakamura4, Hisaaki Nishio5, Isao Nishi6, Keigo Kimura6, Makoto Niki7, Tamotsu Ono8, Noriyuki Sueyoshi9, Machiko Kita9, Kaneyuki Kida10, Masanobu Ohama10, Kaori Satoh11, Hirofumi Toda11, Tetsu Mizutani12, Nozomi Fukuda12, Kana Sawa12, Isako Nakai13, Tomomi Kofuku13, Tamaki Orita14, Hideo Watari15, Satoshi Shimura15, Saori Fukuda16, Akihiro Nakamura2, Yasunao Wada17.
Abstract
Carbapenemase-producing Enterobacteriaceae (CPE) are increasing worldwide. Rapid and accurate detection of CPE is necessary for appropriate antimicrobial treatment and hospital infection control. However, CPE contains some strains that are difficult to detect depending on genotype and MIC value of carbapenem, and a detection method has not been established. The recently reported modified carbapenem inactivation method (mCIM) has been developed in CLSI M100-S27 as a phenotypic technique for detecting carbapenemase activity. In the present study, we examined mCIM as a new CPE detection method using 207 Enterobacteriaceae isolates in comparison with the three existing screening methods of modified Hodge test, Carba NP test and carbapenem inactivation method and evaluated its performance. Consequently, both the sensitivity and specificity of mCIM were 100%, indicating better results than the conventional screening methods. The mCIM is a useful tool for microbiology laboratories due to its simplicity, clear criteria, cost-effectiveness and availability at any laboratory.Entities:
Keywords: Carba NP test; Carbapenemase-producing Enterobacteriaceae; Modified Hodge test; Modified carbapenem inactivation method test
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Year: 2017 PMID： 29248418 DOI： 10.1016/j.jiac.2017.11.010
Source DB: PubMed Journal: J Infect Chemother ISSN： 1341-321X Impact factor: 2.211