| Literature DB >> 29155757 |
Thiago Cabral1, Marcus A Toral2, Gabriel Velez2, James E DiCarlo3, Anuradha M Gore4, MaryAnn Mahajan4, Stephen H Tsang3, Alexander G Bassuk5, Vinit B Mahajan6.
Abstract
The human retina is composed of the sensory neuroretina and the underlying retinal pigmented epithelium (RPE), which is firmly complexed to the vascular choroid layer. Different regions of the retina are anatomically and molecularly distinct, facilitating unique functions and demonstrating differential susceptibility to disease. Proteomic analysis of each of these regions and layers can provide vital insights into the molecular process of many diseases, including Age-Related Macular Degeneration (AMD), diabetes mellitus, and glaucoma. However, separation of retinal regions and layers is essential before quantitative proteomic analysis can be accomplished. Here, we describe a method for dissection and collection of the foveal, macular, and peripheral retinal regions and underlying RPE-choroid complex, involving regional punch biopsies and manual removal of tissue layers from a human eye.One-dimensional SDS-PAGE as well as downstream proteomic analysis, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), can be used to identify proteins in each dissected retinal layer, revealing molecular biomarkers for retinal disease.Entities:
Mesh：
Year: 2017 PMID： 29155757 PMCID： PMC5755368 DOI： 10.3791/56203
Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355