Şerban Comşa1, Raluca Amalia Ceaușu1, Roxana Popescu2, Anca Maria Cîmpean3, Marius Raica1. 1. Department of Histology, Angiogenesis Research Center, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania. 2. Department of Cell and Molecular Biology, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania. 3. Department of Histology, Angiogenesis Research Center, "Victor Babeş" University of Medicine and Pharmacy, Timisoara, Romania ancacimpean1972@yahoo.com.
Abstract
BACKGROUND/AIM: To analyze the interaction between the human mesenchymal stem cells (hMSC) and the chick embryo chorioallantoic membrane (CAM), in order to assess the still obscure process of vasculogenesis. MATERIALS AND METHODS: We implanted hMSC onto CAM and we analyzed the morphology and the immunohistochemical profile of CAM. RESULTS: hMSC adhered to CAM, few of them entered the chorionic epithelium and the mesoderm and developed a CD44-/Ki67- status. hMSC stimulated the CAM mesenchymal cells (cMSC) to acquire endothelial and pericyte-like features and to generate cord/capillary-like structures (CLS) in the chorionic epithelium and the mesoderm, but they also entered these structures (CD34+/SMA (smooth muscle actin)+ hMSC). Simultaneously, hMSC induced a process of sprouting angiogenesis in the mesoderm, CD105+ hMSC being identified in the proximity of the angiogenic areas. CONCLUSION: hMSC and CAM establish a genuine hotspot of vasculogenesis, which may evolve to a valuable experimental model for this research field. Copyright
BACKGROUND/AIM: To analyze the interaction between the human mesenchymal stem cells (hMSC) and the chick embryo chorioallantoic membrane (CAM), in order to assess the still obscure process of vasculogenesis. MATERIALS AND METHODS: We implanted hMSC onto CAM and we analyzed the morphology and the immunohistochemical profile of CAM. RESULTS:hMSC adhered to CAM, few of them entered the chorionic epithelium and the mesoderm and developed a CD44-/Ki67- status. hMSC stimulated the CAM mesenchymal cells (cMSC) to acquire endothelial and pericyte-like features and to generate cord/capillary-like structures (CLS) in the chorionic epithelium and the mesoderm, but they also entered these structures (CD34+/SMA (smooth muscle actin)+ hMSC). Simultaneously, hMSC induced a process of sprouting angiogenesis in the mesoderm, CD105+ hMSC being identified in the proximity of the angiogenic areas. CONCLUSION:hMSC and CAM establish a genuine hotspot of vasculogenesis, which may evolve to a valuable experimental model for this research field. Copyright
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