Etiene Andrade Munhoz1, Augusto Bodanezi1, Tania Mary Cestari Biol2, Márcia Sirlene Zardin Graeff3, Osny Ferreira Junior4, Paulo Sergio Perry de Carvalho5, Rumio Taga6. 1. Assistant Professor, Department of Dentistry, Health Science Centre, Federal University of Santa Catarina, Florianópolis, Brazil. 2. Scientific Laboratory Technician, Department of Oral Biology, School of Dentistry, University of São Paulo, Bauru, Brazil. 3. Scientific Laboratory Technician, Integrated Research Center, Dental School of Bauru, University of São Paulo, São Paulo, Bauru, Brazil. 4. Associate Professor, Department of Stomatology, Radiology and Oral Surgery, School of Dentistry, University of São Paulo, Bauru, Brazil. 5. Chairman in Oral Surgery, Department of Stomatology, Radiology and Oral Surgery, School of Dentistry, University of São Paulo, Bauru, Brazil. 6. Chairman in Oral Biology, Department of Oral Biology, School of Dentistry, University of São Paulo, Bauru, Brazil.
Abstract
PURPOSE: To evaluate if an inorganic graft applied before implant insertion interferes with osseointegration. MATERIALS AND METHODS: The bilateral mandibular incisors of 12 rabbits were extracted. One of the sockets was randomly filled with an inorganic xenogenic bone graft, whereas the remaining socket was allowed to heal naturally and served as a control. After 60 days, titanium implants were inserted into healing areas. The animals were killed 60 days after. Bone depositions were marked with fluorochrome oxytetracycline, alizarin, and calcein and evaluated using confocal laser scanning microscopy. Bone-to-implant contact (BIC) and bone area (BA) within the limits of the implant threads were analyzed. Data were compared statically by paired t tests, one-way ANOVA, and Bonferroni post hoc tests (α = 0.05). RESULTS: No differences between the control and experimental groups in bone deposition for each marker, in either the BIC or BA analysis were observed. The bone deposition marked by alizarin (14-21 days) was the highest, followed by oxytetracycline (0 and 7 days) and calcein (45 and 52 days) in both groups (P < 0.05). CONCLUSION: The bone healing or the course of osseointegration was not impaired by the use of an inorganic xenogenic graft before insertion of a titanium implant.
PURPOSE: To evaluate if an inorganic graft applied before implant insertion interferes with osseointegration. MATERIALS AND METHODS: The bilateral mandibular incisors of 12 rabbits were extracted. One of the sockets was randomly filled with an inorganic xenogenic bone graft, whereas the remaining socket was allowed to heal naturally and served as a control. After 60 days, titanium implants were inserted into healing areas. The animals were killed 60 days after. Bone depositions were marked with fluorochrome oxytetracycline, alizarin, and calcein and evaluated using confocal laser scanning microscopy. Bone-to-implant contact (BIC) and bone area (BA) within the limits of the implant threads were analyzed. Data were compared statically by paired t tests, one-way ANOVA, and Bonferroni post hoc tests (α = 0.05). RESULTS: No differences between the control and experimental groups in bone deposition for each marker, in either the BIC or BA analysis were observed. The bone deposition marked by alizarin (14-21 days) was the highest, followed by oxytetracycline (0 and 7 days) and calcein (45 and 52 days) in both groups (P < 0.05). CONCLUSION: The bone healing or the course of osseointegration was not impaired by the use of an inorganic xenogenic graft before insertion of a titanium implant.
Authors: Nathália R Gomes; Juliano D S Albergaria; Jonathas A de S Henriques; Tânia M P Amaral; Evandro N Abdo; Gerluza A B Silva; Cláudia Borges Brasileiro Journal: Dentomaxillofac Radiol Date: 2019-07-23 Impact factor: 2.419