Fan Pu1,2, Wenpeng Zhang3, Kevin P Bateman4, Yong Liu5, Roy Helmy5, Zheng Ouyang1,3. 1. State Key Laboratory of Precision Measurement Technology & Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, PR China. 2. Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. 3. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA. 4. Pharmacokinetics, Pharmacodynamics & Drug Metabolism, Merck & Co., Incorporated, West Point, PA 19486, USA. 5. Merck Research Laboratories, PO Box 2000, RY818-C220, Rahway, NJ 07065, USA.
Abstract
AIM: Preclinical pharmacokinetic studies are an essential part of modern drug development. In this work, we explored a new solution with onsite analysis using a miniature MS system, which can significantly improve the efficiency of the preclinical study. Materials & methods: A miniature mass spectrometer was used with an automatic blood sampler for onsite quantitation of drug compounds in whole blood samples. Slug-flow microextraction was used to replace the in-lab sample preparation. RESULTS & CONCLUSION: Animal studies were carried out using two drug compounds, using the auto sampler to take blood samples at preprogrammed time points. The miniature MS system was used to obtain drug concentrations, which were subsequently used to calculate the pharmacokinetic parameters.
AIM: Preclinical pharmacokinetic studies are an essential part of modern drug development. In this work, we explored a new solution with onsite analysis using a miniature MS system, which can significantly improve the efficiency of the preclinical study. Materials & methods: A miniature mass spectrometer was used with an automatic blood sampler for onsite quantitation of drug compounds in whole blood samples. Slug-flow microextraction was used to replace the in-lab sample preparation. RESULTS & CONCLUSION: Animal studies were carried out using two drug compounds, using the auto sampler to take blood samples at preprogrammed time points. The miniature MS system was used to obtain drug concentrations, which were subsequently used to calculate the pharmacokinetic parameters.
Entities:
Keywords:
automatic blood sampler; miniature mass spectrometer; preclinical pharmacokinetics
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