Isaac L Moss1, Yejia Zhang, Peng Shi, Ana Chee, Marge J Piel, Howard S An. 1. Department of Orthopaedic Surgery, New England Musculoskeletal Institute, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030, USA. imoss@uchc.edu
Abstract
BACKGROUND CONTEXT: The rabbit annular puncture model of degeneration is among the most widely used models of intervertebral disc (IVD) degeneration. There are no published reports of the specific surgical technique used to produce this model. PURPOSE: To describe the model in detail in an effort to reduce center-to-center variability and hopefully improve the reproducibility of future experimental results. STUDY DESIGN: Technical report of surgical approach and experience. PATIENT SAMPLE: New Zealand White Rabbits. METHODS: A detailed report of the annular puncture technique in rabbits is provided including preparation of the animals, instrumentation, a description of retroperitoneal approach to the lumbar area, and the technique for IVD injury. Common pitfalls and complications of the procedure are described. CONCLUSIONS: The methods described can serve to standardize the implementation of this important preclinical model of disc degeneration.
BACKGROUND CONTEXT: The rabbit annular puncture model of degeneration is among the most widely used models of intervertebral disc (IVD) degeneration. There are no published reports of the specific surgical technique used to produce this model. PURPOSE: To describe the model in detail in an effort to reduce center-to-center variability and hopefully improve the reproducibility of future experimental results. STUDY DESIGN: Technical report of surgical approach and experience. PATIENT SAMPLE: New Zealand White Rabbits. METHODS: A detailed report of the annular puncture technique in rabbits is provided including preparation of the animals, instrumentation, a description of retroperitoneal approach to the lumbar area, and the technique for IVD injury. Common pitfalls and complications of the procedure are described. CONCLUSIONS: The methods described can serve to standardize the implementation of this important preclinical model of disc degeneration.
Authors: Stefan Schwan; Christopher Ludtka; Ingo Wiesner; Andre Baerthel; Andrea Friedmann; Felix Göhre Journal: Eur Spine J Date: 2017-10-27 Impact factor: 3.134
Authors: John T Martin; Christopher M Collins; Kensuke Ikuta; Robert L Mauck; Dawn M Elliott; Yeija Zhang; D Greg Anderson; Alexander R Vaccaro; Todd J Albert; Vincent Arlet; Harvey E Smith Journal: J Orthop Res Date: 2014-10-01 Impact factor: 3.494
Authors: Yejia Zhang; Ana Chee; Peng Shi; Rui Wang; Isaac Moss; Er-Yun Chen; Tong-Chuan He; Howard S An Journal: Am J Phys Med Rehabil Date: 2015-07 Impact factor: 2.159