| Literature DB >> 29039418 |
Ingo Gregor1,2, Martin Spiecker1, Roman Petrovsky3, Jörg Großhans3, Robert Ros4,5,6, Jörg Enderlein1,2.
Abstract
Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.Mesh:
Year: 2017 PMID: 29039418 DOI: 10.1038/nmeth.4467
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547