Fahimeh Rezazadeh1, Rahele Ebrahimi2, Azadeh Andisheh-Tadbir3, Mohammad Javad Ashraf4, Bijan Khademi5. 1. Oral and Dental Disease Research Center, Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Shahrekord University of Medical Sciences, Shahrekord, Iran. 3. Oral and Dental Disease Research Center, Dept. of Oral & Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Dept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Otolaryngology Research Center, Dept. of Otorhinolaryngology, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
STATEMENT OF THE PROBLEM: Squamous cell carcinoma (SCC) is the most frequent oral cancer whose 5-year survival rate is 80% for early-detected lesions and nearly 30-50% for advanced lesions. Early detection of oral cancers and precancerous lesions can improve the patient's survival and decrease the morbidity. PURPOSE: This study aimed to evaluate and compare the Ki-67 and MCM3 expression in cytologic smear of oral SCC (OSCC). MATERIALS AND METHOD: We examined 48 oral brush biopsies including 28 OSCC and 20 healthy non-smoking samples. Immunocytochemistry staining was performed for Ki-67 and MCM3 by using an EnVision-labeled peroxidase system, and labeling index (LI) was calculated. RESULTS: Out of 28 OSCC cases, 27(96.4%) cases contained MCM3 positive cells and 22(78%) cases contained Ki-67 positive cells. All normal mucosa were Ki-67 and MCM3 negative. MCM3 and Ki-67 LI were significantly higher in OSCC than normal mucosa (p< 0.001). MCM3 LI was significantly higher than Ki-67 LI in OSCC group (p< 0.001). CONCLUSION: Immunocytologic evaluation of Ki-67 and MCM3 can be used for early detection of OSCC. Furthermore, MCM3 may be a more sensitive cytologic biomarker than Ki-67 in SCC patients.
STATEMENT OF THE PROBLEM: Squamous cell carcinoma (SCC) is the most frequent oral cancer whose 5-year survival rate is 80% for early-detected lesions and nearly 30-50% for advanced lesions. Early detection of oral cancers and precancerous lesions can improve the patient's survival and decrease the morbidity. PURPOSE: This study aimed to evaluate and compare the Ki-67 and MCM3 expression in cytologic smear of oral SCC (OSCC). MATERIALS AND METHOD: We examined 48 oral brush biopsies including 28 OSCC and 20 healthy non-smoking samples. Immunocytochemistry staining was performed for Ki-67 and MCM3 by using an EnVision-labeled peroxidase system, and labeling index (LI) was calculated. RESULTS: Out of 28 OSCC cases, 27(96.4%) cases contained MCM3 positive cells and 22(78%) cases contained Ki-67 positive cells. All normal mucosa were Ki-67 and MCM3 negative. MCM3 and Ki-67 LI were significantly higher in OSCC than normal mucosa (p< 0.001). MCM3 LI was significantly higher than Ki-67 LI in OSCC group (p< 0.001). CONCLUSION: Immunocytologic evaluation of Ki-67 and MCM3 can be used for early detection of OSCC. Furthermore, MCM3 may be a more sensitive cytologic biomarker than Ki-67 in SCCpatients.
Authors: Zohreh Jaafari Ashkavandi; Ali Dehghani Najvani; Azadeh Andishe Tadbir; Soheil Pardis; Mohammad Ali Ranjbar; Mohammad Javad Ashraf Journal: Asian Pac J Cancer Prev Date: 2013
Authors: I S Scott; E Odell; P Chatrath; L S Morris; R J Davies; S L Vowler; R A Laskey; N Coleman Journal: Br J Cancer Date: 2006-04-24 Impact factor: 7.640