| Literature DB >> 28935668 |
Shangtao Cao1,2,3, Shengyong Yu1,2, Yan Chen1,2, Xiaoshan Wang1,2,3,4, Chunhua Zhou1,2,3, Yuting Liu1,2, Junqi Kuang1,2,3, He Liu1,2,3, Dongwei Li1,2,3,4, Jing Ye1,2, Yue Qin1,2,3, Shilong Chu1,2, Linlin Wu1,2,3, Lin Guo1,2, Yinxiong Li1,2, Xiaodong Shu1,2, Jiekai Chen1,2,4, Jing Liu5,2, Duanqing Pei6,2,4.
Abstract
We report here an approach to redirecting somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts to epithelial-like cells with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting epithelial-like cells that can be further reprogrammed to endodermal progenitor cells. Interestingly, these cells can self-renew in vitro and further differentiate into albumin-producing hepatocytes that can rescue mice from acute liver injury. Our results demonstrate a rational approach to convert mouse embryonic fibroblasts to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells.Entities:
Keywords: MET; cell signaling; chemical biology; endoderm progenitor cells; hepatocyte; reprogramming; small molecule
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Year: 2017 PMID： 28935668 PMCID： PMC5704493 DOI： 10.1074/jbc.M117.812537
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157