Literature DB >> 2889700

Synthetic oligonucleotide probes complementary to rRNA for group- and species-specific detection of mycoplasmas.

U Göbel1, R Maas, G Haun, C Vinga-Martins, E J Stanbridge.   

Abstract

A variety of genetic probes has been used for the detection of pathogenic bacteria. Here we present a straightforward approach for the development of group- and species-specific oligonucleotide probes complementary to mycoplasmal rRNA. These probes are useful not only for the identification of mycoplasmas in clinical specimens, but also for the detection of Mollicutes in contaminated cell cultures. Radiolabeled rDNA probes detected less than 1 x 10(3) organisms in an RNA-DNA hybridization procedure.

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Year:  1987        PMID: 2889700

Source DB:  PubMed          Journal:  Isr J Med Sci        ISSN: 0021-2180


  8 in total

1.  High-density microarray of small-subunit ribosomal DNA probes.

Authors:  Kenneth H Wilson; Wendy J Wilson; Jennifer L Radosevich; Todd Z DeSantis; Vijay S Viswanathan; Thomas A Kuczmarski; Gary L Andersen
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

2.  Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.

Authors:  K H Wilson; R B Blitchington; R C Greene
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

3.  Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction.

Authors:  K H Wilson; R Blitchington; P Shah; G McDonald; R D Gilmore; L P Mallavia
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

4.  Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.

Authors:  U Edwards; T Rogall; H Blöcker; M Emde; E C Böttger
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971

5.  Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

Authors:  A Meier; D H Persing; M Finken; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

6.  rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

Authors:  G R Reddy; J B Dame
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

7.  Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species.

Authors:  K H Wilson; R Blitchington; B Hindenach; R C Greene
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

8.  Detection of Mycoplasma hyopneumoniae by using rRNA-oligodeoxynucleotide hybridization.

Authors:  S Futo; Y Seto; S Mitsuse; Y Mori
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

  8 in total

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