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Literature DB >> 1629339

rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

Abstract

Three synthetic oligonucleotide probes complementary to unique regions of Babesia bigemina small-subunit rRNA were developed for detecting the parasite in bovine blood. These probes specifically detected a parasitemia of 2 x 10(-5)% by autoradiography in less than 24 h by using a 200-microliters sample of bovine blood. These probes did not bind to total RNA or genomic DNA isolated from another closely related species, Babesia bovis, or to bovine leukocyte RNA. This method detected B. bigemina infections in calves inoculated with as few as 1,000 infected erythrocytes from the second day onward for 16 days.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1992 PMID： 1629339 PMCID： PMC265386 DOI： 10.1128/jcm.30.7.1811-1814.1992

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

19 in total

1. Ribosomal RNA: nature's own polymerase-amplified target for diagnosis.

Authors: A P Waters; T F McCuthan
Journal: Parasitol Today Date: 1990-02

2. Oligodeoxynucleotide probes for Campylobacter fetus and Campylobacter hyointestinalis based on 16S rRNA sequences.

Authors: I V Wesley; R D Wesley; M Cardella; F E Dewhirst; B J Paster
Journal: J Clin Microbiol Date: 1991-09 Impact factor: 5.948

3. Compilation of small ribosomal subunit RNA sequences.

Authors: E Dams; L Hendriks; Y Van de Peer; J M Neefs; G Smits; I Vandenbempt; R De Wachter
Journal: Nucleic Acids Res Date: 1988 Impact factor: 16.971

4. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors: P Chomczynski; N Sacchi
Journal: Anal Biochem Date: 1987-04 Impact factor: 3.365

5. Concentration and enzyme content of in vitro-cultured Babesia bigemina-infected erythrocytes.

Authors: C A Vega; G M Buening; S D Rodriguez; C A Carson
Journal: J Protozool Date: 1986-11

6. Rapid identification of Mycobacterium avium complex in culture using DNA probes.

Authors: T A Drake; J A Hindler; O G Berlin; D A Bruckner
Journal: J Clin Microbiol Date: 1987-08 Impact factor: 5.948

7. Detection of Babesia bovis using DNA hybridization.

Authors: G L McLaughlin; T D Edlind; G M Ihler
Journal: J Protozool Date: 1986-02

8. Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture.

Authors: G R Reddy; D Chakrabarti; C A Yowell; J B Dame
Journal: Nucleic Acids Res Date: 1991-07-11 Impact factor: 16.971

rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

1. Ribosomal RNA: nature's own polymerase-amplified target for diagnosis.

2. Oligodeoxynucleotide probes for Campylobacter fetus and Campylobacter hyointestinalis based on 16S rRNA sequences.

3. Compilation of small ribosomal subunit RNA sequences.

4. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

5. Concentration and enzyme content of in vitro-cultured Babesia bigemina-infected erythrocytes.

6. Rapid identification of Mycobacterium avium complex in culture using DNA probes.

7. Detection of Babesia bovis using DNA hybridization.

8. Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture.

9. Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes.

10. DNA probes for the detection of Babesia caballi.

1. Monitoring Babesia bovis infections in cattle by using PCR-based tests.

Review 2. The role of nuclear technologies in the diagnosis and control of livestock diseases--a review.