Different methodologies for sustainability of optimization techniques used in submerged and solid state fermentation.

Optimization techniques are considered as a part of nature's way of adjusting to the changes happening around it. There are different factors that establish the optimum working condition or the production of any value-added product. A model is accepted for a particular process after its sustainability has been verified on a statistical and analytical level. Optimization techniques can be divided into categories as statistical, nature inspired and artificial neural network each with its own benefits and usage in particular cases. A brief introduction about subcategories of different techniques that are available and their computational effectivity will be discussed. The main focus of the study revolves around the applicability of these techniques to any particular operation such as submerged fermentation (SmF) and solid state fermentation (SSF), their ability to produce secondary metabolites and the usefulness in the laboratory and industrial level. Primary studies to determine the enzyme activity of different microorganisms such as bacteria, fungi and yeast will also be discussed. l-Asparaginase, the most commonly used drugs in the treatment of acute lymphoblastic leukemia (ALL) shall be considered as an example, a short discussion on models used in the production by the processes of SmF and SSF will be discussed to understand the optimization techniques that are being dealt. It is expected that this discussion would help in determining the proper technique that can be used in running any optimization process for different purposes, and would help in making these processes less time-consuming with better output.