Nanocarriers-based immobilization of enzymes for industrial application.

Nanocarriers-based immobilization strategies are a novel concept in the enhancement of enzyme stability, shelf life and efficiency. A wide range of natural and artificial supports have been assessed for their efficacy in enzyme immobilization. Nanomaterials epitomize unique and fascinating matrices for enzyme immobilization. These structures include carbon nanotubes, superparamagnetic nanoparticles and nanofibers. These nano-based supports offer stable attachment of enzymes, thus ensuring their reusability in diverse industrial applications. This review attempts to encompass recent developments in the critical role played by nanotechnology towards the improvement of the practical applicability of microbial enzymes. Nanoparticles are increasingly being used in combination with various polymers to facilitate enzyme immobilization. These endeavors are proving to be conducive for enzyme-catalyzed industrial operations. In recent years the diversity of nanomaterials has grown tremendously, thus offering endless opportunities in the form of novel combinations for various biotransformation experimentations. These nanocarriers are advantageous for both free enzymes and whole-cell immobilization, thus demonstrating to be relatively effective in several fermentation procedures. © King Abdulaziz City for Science and Technology 2021.