Purpose: Optical coherence tomography's (OCT) third outer retinal band has been attributed to the zone of interdigitation between RPE cells and cone outer segments. The purpose of this paper is to investigate the structure of this band with adaptive optics (AO)-OCT. Methods: Using AO-OCT, images were obtained from two subjects. Axial structure was characterized by measuring band 3 thickness and separation between bands 2 and 3 in segmented cones. Lateral structure was characterized by correlation of band 3 with band 2 and comparison of their power spectra. Band thickness and separation were also measured in a clinical OCT image of one subject. Results: Band 3 thickness ranged from 4.3 to 6.4 μm. Band 2 correlations ranged between 0.35 and 0.41 and power spectra of both bands confirmed peak frequencies that agree with histologic density measurements. In clinical images, band 3 thickness was between 14 and 19 μm. Measurements of AO-OCT of interband distance were lower than our corresponding clinical OCT measurements. Conclusions: Band 3 originates from a structure with axial extent similar to a single surface. Correlation with band 2 suggests an origin within the cone photoreceptor. These two observations indicate that band 3 corresponds predominantly to cone outer segment tips (COST). Conventional OCT may overestimate both the thickness of band 3 and outer segment length.
Purpose: Optical coherence tomography's (OCT) third outer retinal band has been attributed to the zone of interdigitation between RPE cells and cone outer segments. The purpose of this paper is to investigate the structure of this band with adaptive optics (AO)-OCT. Methods: Using AO-OCT, images were obtained from two subjects. Axial structure was characterized by measuring band 3 thickness and separation between bands 2 and 3 in segmented cones. Lateral structure was characterized by correlation of band 3 with band 2 and comparison of their power spectra. Band thickness and separation were also measured in a clinical OCT image of one subject. Results: Band 3 thickness ranged from 4.3 to 6.4 μm. Band 2 correlations ranged between 0.35 and 0.41 and power spectra of both bands confirmed peak frequencies that agree with histologic density measurements. In clinical images, band 3 thickness was between 14 and 19 μm. Measurements of AO-OCT of interband distance were lower than our corresponding clinical OCT measurements. Conclusions: Band 3 originates from a structure with axial extent similar to a single surface. Correlation with band 2 suggests an origin within the cone photoreceptor. These two observations indicate that band 3 corresponds predominantly to cone outer segment tips (COST). Conventional OCT may overestimate both the thickness of band 3 and outer segment length.
Authors: Yan Zhang; Barry Cense; Jungtae Rha; Ravi S Jonnal; Weihua Gao; Robert J Zawadzki; John S Werner; Steve Jones; Scot Olivier; Donald T Miller Journal: Opt Express Date: 2006-05-15 Impact factor: 3.894
Authors: Athanasios Panorgias; Robert J Zawadzki; Arlie G Capps; Allan A Hunter; Lawrence S Morse; John S Werner Journal: Invest Ophthalmol Vis Sci Date: 2013-06-26 Impact factor: 4.799
Authors: Vivek J Srinivasan; Bryan K Monson; Maciej Wojtkowski; Richard A Bilonick; Iwona Gorczynska; Royce Chen; Jay S Duker; Joel S Schuman; James G Fujimoto Journal: Invest Ophthalmol Vis Sci Date: 2008-04 Impact factor: 4.799
Authors: Omer P Kocaoglu; Zhuolin Liu; Furu Zhang; Kazuhiro Kurokawa; Ravi S Jonnal; Donald T Miller Journal: Biomed Opt Express Date: 2016-10-13 Impact factor: 3.732
Authors: Glenn Yiu; Zhe Wang; Christian Munevar; Eric Tieu; Bradley Shibata; Brittany Wong; David Cunefare; Sina Farsiu; Jeffrey Roberts; Sara M Thomasy Journal: Exp Eye Res Date: 2018-01-17 Impact factor: 3.467
Authors: Benjamin S Sajdak; Brent A Bell; Tylor R Lewis; Gabriel Luna; Grayson S Cornwell; Steven K Fisher; Dana K Merriman; Joseph Carroll Journal: Invest Ophthalmol Vis Sci Date: 2018-05-01 Impact factor: 4.799
Authors: Mehdi Azimipour; Robert J Zawadzki; Iwona Gorczynska; Justin Migacz; John S Werner; Ravi S Jonnal Journal: PLoS One Date: 2018-10-25 Impact factor: 3.240