Transformation products elucidation of forchlorfenuron in postharvest kiwifruit by time-of-flight mass spectrometry.

Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea, FCF) is a plant growth regulator, being extensively used for increasing kiwifruit size. The toxicological properties of its may persist in their transformation products (TPs) or even higher toxicity than FCF. TPs elucidation of FCF in postharvest kiwifruit (Actinidia chinensis, Chinese gooseberry) by the liquid chromatography ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) in positive mode was the objective of the present study. Fifteen days after full bloom, kiwifruits were dipped for 5s with high dosage FCF solution (60 mg/L), so that sufficient peaks could be detected. The chemical structure of unknown TPs was analyzed in combination of functions of LCMS-IT-TOF, such as high-accurate MSn, formula predictor, metabolite structural analysis software MetID Solution, profiling solution metabolomics software, and neutral loss, characteristic isotopic patterns of chlorine, the fragmentation pattern and retention time of standard substances, nitrogen rule, chemical components of kiwifruit. Total 17 TPs were detected via comparisons of their accurate MSn data of commercial analytical standards and synthesized standards with high purity, such as 4-amino-2-chloropyridine, phenylurea, 2-hydroxy-FCF, 1-(2-chloro-6-((3, 4, 5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl) oxy) pyridin-4-yl)-3-phenylurea, 1, 3-bis (2-chloropyridin-4-yl) urea, 1,3-diphenylurea, 1-(2-chloropyridin-4-yl)urea, FCF-2-O-β-D-glucoside, and so on. The major transformation pathways of FCF in kiwifruit were biochemical and photochemical cleavage pathway. The experimental results indicate that LCMS-IT-TOF is powerful and effective tool for identification of FCF TPs.

Introduction

FCF is a plant growth regulator, being extensively used for increasing fruit size and weight in watermelon, kiwifruit, grape and apple[1-4]. In China, the incidence of FCF had caused an adverse impact on the kiwifruit industry. Residue analysis and dissipation of FCF in fruit and vegetable had already been studied by high-performance liquid chromatography with ultraviolet detection (HPLC/UV)[5], liquid chromatography-tandem mass spectrometry[6-8], liquid chromatography time-of-flight mass spectrometry (LC/TOF-MS)[9]. An enzyme-linked immunosorbent assay (ELISA) had been developed for the determination of FCF in fruit[10-12]. Evaluation of the new activity of FCF in the product was reported by Australian pesticides and veterinary medicines authority. FCF has low oral toxicity in rats with an LD50 of 4940 mg/kg bw in male rats and 4899 mg/kg bw in females[13]. In recent years, some researches indicated that TPs of agrochemistry could be more toxic than the parent molecule[14,15]. So FCF and its TPs could be a potential health hazard. However, the detection of TPs was a difficult work to do, due to the lack of standards[16]. Currently, the time-of-flight (TOF) mass analyzers had been used to detect TPs of agrochemistry[17-21].

LCMS-IT-TOF (Shimadzu) is a type of mass spectrometer that combines ion trap and TOF (time-of-flight) technologies. The instrument possesses some advantages and function, such as high accuracy MSn, Formula Predictions software, MetID solution software[22]. LCMS-IT-TOF/MS had been successfully applied to the identification of metabolites of FR429[23], herbal homologs, strictosamide, phencynonate[24,25],. In this paper, LCMS-IT-TOF had been employed to identify the major TPs of FCF in postharvest kiwifruit.

Material and methods

Ethics statement

I state clearly that no specific permissions were required for these locations/activities, because this site is a normal kiwifruit orchard without protected wildlife and protected area of land or sea. The authors confirm that the field studies did not involve endangered or protected species. The study was approved by Shaanxi Bairui Kiwi Fruit Research Institute Co. Ltd.

FCF (purity>99%, M0), 4-amino-2-chloropyridine (97%, ST1), phenylurea (97%, ST2) were provided by Sigma-Aldrich. FCF-4-O-β-D-glucoside (>95%, ST3), FCF-3- O-β-D -glucoside (>95%, ST4), 4-hydroxy-FCF (>95%, ST5) and 3-hydroxy-FCF (>95%, ST6) come from chemical synthesis [28]. High performance liquid chromatography (HPLC) grade methanol and acetonitrile were provided by Sigma-Aldrich. Formic acid and ethyl acetate were of analytical grade. HPLC grade water was obtained in a Milli-Q-Plus ultrapure water system from Millipore.

Plant material

The trial was carried out in Xi'an city, Shaanxi province, China, in 2013~2015, in a 7-year-old kiwifruit orchard of Hayward from Shaanxi Bairui Kiwi Fruit Research Institute Co. Ltd (34°03′N, 108°25′E). The vines were spaced 5×4 m, trained to the T-bar trellis system. Twenty vines were selected, uniform in vegetative and reproductive characteristics. Fifteen days after full bloom, all fruitlets of fifteen vines were dipped for 5 s with FCF (ethanol solvent, 60 mg/L), sufficient peaks could be detected in high dosage, while the fruit of the other five vines were dipped with water only (blank). Storage time of postharvest kiwifruit is one month.

Sample preparation

Extraction of FCF and TPs from kiwifruit samples was carried out according to the QuEChERS method[26,27]: (1) FCF treated samples were placed in a blender and chopped. (2) 300 g of thoroughly homogenized sample was placed into a 1000 mL glass beaker, mixed thoroughly with 300 mL of acetonitrile and 120 g anhydrous magnesium sulfate, and 30 g anhydrous sodium acetate using ultrasonic-assisted extraction for 40 min. The homogenate was allowed to settle and the supernatant was filtered through a filter paper into a 1000 mL rotary-evaporation flask. The solid residue was washed twice with 60 mL of acetonitrile. A rotary evaporator set at 50°C and 250 mbar was used to evaporate the extract to less than 5 mL, and then, the extract was passed to a graduated conical tube (15 mL) and evaporated to dryness at 50°C. The sample was reconstituted in water: acetonitrile (1:1) and filtered through a 0.22 μm filter[28].

Chromatographic conditions

LC analysis was conducted on a LC-20AD system from Shimadzu (Kyoto, Japan). Chromatographic separation was achieved on a Shim-pack XR ODS column (2.2μm 3.0 mm×75 mm from Shimadzu). The mobile phase (0.2 mL min-1) consisted of solvent A (0.1% formic acid in acetonitrile) and B (0.1% formic acid in water). Elution condition was performed with a linear gradient 95~30% B from 0 to 5 min, 30~0% B from 5 to10 min, retained until 1 min then quickly returned to initial 95% B and maintained for 20 min for column balance [28].

MSn analyses were conducted on a Shimadzu LC-IT-TOF/MS (Shimadzu, Kyoto, Japan) equipped with an electrospray ionization (ESI) source operated in positive mode, and the optimized operating conditions were as follows: detector voltage, 1.6 kV; curved desolvation line (CDL) temperature, 200°C; heat block temperature, 200°C; nebulizing gas flow, 1.5 L/min; drying gas (N2) pressure, 100kPa; scan range, m/z 100–1000 for MS1, m/z 100–800 for MS2, m/z 100–800 for MS3 [].

The identification process of FCF and its TPs involves four procedural steps: (1) sample preparation (2) elucidated the fragment pattern of FCF and its 6 TPs standard substance. (3)The chemical structure of unknown TPs was analyzed in combination of functions of LCMS-IT-TOF, such as high-accurate MSn, formula predictor, metabolite structural analysis software MetID Solution, profiling solution metabolomics software, and neutral loss, characteristic isotopic patterns of chlorine, the fragmentation pattern and retention time of standard substances, nitrogen rule, chemical components of kiwifruit. Some suspected TPs peaks which had the same MSn and fragment pattern with FCF and its 6 TPs standard substance were found by full scan analysis in positive mode. (4) concluded their chemistry structure.

Results and discussion

Fragmentation mass spectra for standard substances of (possible) transformation products

Forchlorfenuron (M0) and 6 TPs (10 μg mL-1) standard substance prepared respectively in methanol was used for the fragmentation pattern study in positive ion mode. Fig 1 shows extract ion chromatograms of 6 TPs standard substance and M0. Table 1 shows analytical information of the 6 TPs standard substance, including retention time, elemental compositions, and mass error in positive ion mode. Fig 2 shows the proposed fragmentation pattern of M0 and 6 TPs standard substance [28].

Fig 1

Extract ion chromatograms of 6 TPs standard substance and M0.

Table 1

The MSn data of the 6 TPs and M0 in positive ion mode.

ST	RT(min)	Theoretical Value [M+H]+	Elementalcompositions	MSn	MSn[M+H]+	Error(ppm)
ST1	2.003	129.0214	C5H5ClN2	MS1	129.0207	-5.4
				MS2	93.0492
ST2	4.560	137.0709	C7H8N2O	MS1	137.0705	-2.9
				MS2	94.0688
ST3	4.470	426.1063	C18H20ClN3O7	MS1	426.1045	-4.2
				MS2	264.0514, 155.0004,129.0162
				MS3	154.9999, 129.0218, 110.0563, 93.0441
ST4	4.710	426.1063	C18H20ClN3O7	MS1	426.1035	-6.6
				MS2	264.0515, 154.9991, 129.0213
				MS3	155.0008, 129.0212, 110.0563, 93.0436
ST5	5.370	264.0534	C12H10ClN3O2	MS1	264.0514	-7.6
				MS2	154.9992, 129.0203, 110.0575, 93.0419
ST6	5.670	264.0534	C12H10ClN3O2	MS1	264.0515	-7.2
				MS2	154.9986, 129.0215, 110.0605, 93.0429
M0	6.660	248.0585	C12H10ClN3O	MS1	248.0572	-5.2
				MS2	155.0001, 129.0208, 93.0436

Standard Substance = ST, retention time = RT

Fig 2

The proposed fragmentation pattern of M0 and 6 TPs standard substance.

Identification of forchlorfenuron transformation products in postharvest kiwifruit

One month after kiwifruit posthavest, 17 TPs were deduced by analysis of LCMS-IT-TOF. Fig 3 shows extract ion chromatograms of 17 TPs. Table 2 displays the data of the MSn data of 17 TPs. Fig 4A–4G show TOF-MS/MS spectrum on TPs and their proposed structures in positive ion mode.

Fig 3

Extract ion chromatograms of 17 TPs.

Table 2

The MSn data of the 17 TPs.

TPs	RT(min)	Elementalcompositions	MSn	MSn[M+H]+	Error(ppm)
TP1	2.001	C5H5ClN2	MS1	129.0214	0
			MS2	93.0441	-6.5
TP2	4.085	C6H6ClN3O	MS1	172.0280	4.7
			MS2	155.0012, 129.0219	3.2, 3.9
TP3	4.467	C18H20ClN3O7	MS1	426.1080	4.0
			MS2	264.0553, 154.9995, 129.0219	7.2, -7.7, 3.9
			MS3	155.0014, 129.0225, 110.0610, 93.0445	4.5, 8.5, 9.1, -2.2
TP4	4.563	C7H8N2O	MS1	137.0705	-2.9
			MS2	94.0646	-5.3
TP5	4.900	C13H12N2O2	MS1	229.0961	-4.8
			MS2	110.0606, 94.0649	5.5, -2.1
TP6	5.355	C18H20ClN3O7	MS1	426.1087	5.6
			MS2	264.0539, 248.0591, 155.0000	1.9, 2.4, -4.5
			MS3	155.0004, 129.0211	-1.9, -2.3
TP7	5.363	C13H12N2O2	MS1	229.0957	-6.5
			MS2	110.0593	-6.4
TP8	5.370	C12H10ClN3O2	MS1	264.0525	-3.4
			MS2	155.0022, 129.0219, 93.0441	9.7, -3.9, -6.5
			MS3	93.0439	-8.6
TP9	5.610	C18H20ClN3O7	MS1	426.1045	-4.2
			MS2	307.0664, 264.0560, 145.0169	-8.8, 9.8, 4.1
			MS3	145.0175	8.3
TP10	5.668	C12H10ClN3O2	MS1	264.0558	9.1
			MS2	155.0000, 129.0206	-4.5, -6.2
TP11	5.767	C13H12N2O2	MS1	229.0967	-2.2
			MS2	110.0595, 94.0646	-4.5, -5.3
TP12	6.249	C12H10ClN3O2	MS1	264.053	-1.5
			MS2	155.0019, 129.0222, 110.0593	7.7, 6.2, -6.4
TP13	6.269	C11H9Cl2N4O	MS1	283.0125	-8.1
			MS2	154.9998, 129.0208	-5.8, -4.7
TP14	7.030	C13H12ClN4O2	MS1	291.0636	-2.4
			MS2	154.9996, 137.0711	-7.1, 1.5
			MS3	94.0645	-6.4
TP15	6.987	C13H12N2O	MS1	213.1021	-0.5
			MS2	94.0651	0
TP16	7.086	C13H12ClN3O	MS1	262.0744	0.8
			MS2	154.9999, 129.0215	-5.2, 0.8
TP17	8.119	C19H16ClN4O2	MS1	367.0980	6.5
			MS2	248.0574, 213.1036	-4.4, 6.6
			MS3	94.0658	7.4

Retention time = RT

Fig 4

MSn on FCF TPs and their proposed structures in positive ion mode.

The molecular ion of TP1 showed the predominant protonated molecule ion [M+H]+ at m/z 129.0214 and was calculated as C5H5ClN2 (error, 0 ppm) by the Formula Predictor software. The difference of retention time between TP1 and ST1 was 0.002 min. So, TP1 was identified as ST1.

TP2 (see Fig 4A, Table 2) gave rise to the protonated molecule at m/z 172.0280 with a retention time of 4.085 min, and it was calculated as C6H6ClN3O (error, 4.7 ppm) by the Formula Predictor software. The MS2 products ions at m/z 155.0012 (error, 3.2 ppm), m/z 129.0219 (error, 3.9 ppm) were characteristic fragment ions of M0, and the product ion at m/z 155.0012 was formed through neutral losing of NH3. Thus, TP2 was identified as 1-(2-chloropyridin-4-yl)urea. The proposed structure and the fragment pathway for TP2 are shown in Fig 4A.

TP3 (see Fig 4B, Table 2, m/z 426.1080), eluted at 4.467 min, showed a 37Cl signal at m/z 428.1037, two fragment ions of the MS1 at m/z 248.0587, m/z 129.0201, and the fragment ion at m/z 248.0587 was the characteristic ion of M0. The molecular ion of TP3 gave rise to three main MS2 ions at m/z 264.0553(error, 7.2 ppm), m/z 154.9995(error, -7.7 ppm), m/z 129.0219(error, 3.9 ppm), and the fragment ion at m/z 264.0553 could lead to four MS3 product ions at m/z 155.0014(error, 4.5 ppm), m/z 129.0225(error, 8.5 ppm), m/z 110.0610(error, 9.1 ppm), m/z 93.0445(error, -2.2 ppm), also the product ion at m/z 264.0553 was calculated as C12H10ClN3O2 (error,7.2 ppm) by the Formula Predictor software. Thus, the product ion at m/z 426.1068 was concluded as the glycosylation product of M0. TP3 showed the same fragment pathway and retention time as ST3. Therefore, TP3 was identified as ST3.

TP4, eluted at 4.563 min, showed the predominant protonated molecule ion [M+H]+ at m/z 137.0705, the mass error was -2.9 ppm, and the retention time error was 0.003 min. TP4 yielded a main MS2 ions at m/z 94.0646 (error, -5.3 ppm). So, TP4 was identified as ST2.

TP5, TP7, TP11 (Fig 4C, Table 2) showed respectively the protonated molecular ion [M+H]+ at m/z 229.0961(error, -4.8 ppm), m/z 229.0957(error, -6.5 ppm), m/z 229.0967(error, -2.2 ppm), which were respectively 15.9939 Da (O), 15.9935 Da (O), 15.9945 Da (O) higher than the protonated molecule of TP15 (m/z 213.1022). They were calculated as C13H12N2O2 by the Formula Predictor software. TP5, TP7, TP11 shared the similar MS2 fragment ions at m/z 110.0606 (error, 5.5 ppm), m/z 110.0593 (error, -6.4 ppm), m/z 110.0595 (error, -4.5 ppm). TP5, TP11 shared the similar MS2 fragment ions at m/z 94.0649 (error, -2.1 ppm), m/z 94.0646 (error, -5.3 ppm). Thus, TP5, TP7, TP11 were elucidated by the hydroxylation product of TP14. According to the polar order, we could infer hydroxy position on the benzene ring, TP5, TP7, TP11 is respectively 1-(4-hydroxy-phenyl)–3–phenylurea, 1-(3-hydroxy-phenyl)–3–phenylurea, 1-(2-hydroxy-phenyl)–3–phenylurea. The proposed chemical structure and the fragment pathway for TP5, TP7, TP11 are respectively shown in Fig 4C.

TP6 showed the protonated molecular ion [M+H]+ at m/z 426.1087 (see Fig 4B, Table 2, error, 5.6 ppm), eluted at 5.355 min, yielded three MS2 ions at m/z 264.0539(error, 1.84 ppm), m/z 248.0591(error, 2.4 ppm), m/z 155.0000(error, -4.5 ppm), and the product ion at m/z 264.0539 lead to two MS3 ions at m/z 155.0004(error, -1.9 ppm), m/z 129.0211(error, -2.3 ppm). The fragment ions at m/z 264.0539, m/z 248.0591 were calculated respectively as C12H10ClN3O2, C12H10ClN3O by the Formula Predictor software, and the fragment ions at m/z 264.0539 had the same daughter ions at m/z 155.0004/129.0211 with ST5/ST6, and also the MS2 product ion at m/z 264.0539 was formed through neutral losing m/z 162.0523 (C6H10O5). Thus, we concluded TP6 was the glycosylation product of M0. As can be seen in Fig 4B, the MS1 of TP6 yield five fragment ions at m/z 264.0524, m/z 248.0590, m/z 155.0001, m/z 129.0222, m/z 110.0572, the position of glycosylation for TP6 was concluded in the benzene ring. But the retention time of TP6 had the larger difference with ST3/ST4. Thus, we concluded TP6 was isomers of ST3/ST4, and indentified as FCF-2-O-β-D-glucoside. The proposed structure and the fragment pathway for TP6 are shown in Fig 4B.

TP8, TP10 (see Fig 4D, Fig 4E,Table 2) and TP12 exhibited respectively the protonated molecular ion [M+H]+ at m/z 264.0525(error, -3.4 ppm), m/z 264.0558(error, 9.1 ppm), m/z 264.0530(error, -1.5 ppm), which were respectively 15.994 Da, 15.9973 Da, 15.9945 Da (O) higher than the protonated molecule of M0 (m/z 248.0585), and they were calculated as C12H10ClN3O2 according to the accurate mass by the Formula Predictor software. Thus they could be preliminary concluded as the hydroxylation product of M0. TP8, TP10 and TP12 shared the same MS2 fragment ions at m/z 155.0007, m/z 129.0214, m/z 110.0600 with ST5/ST6. The retention time error between TP8 and ST5, TP10 and ST6 were respectively 0min, 0.003min. Therefore, TP8 was identified as ST5, TP10 was identified as ST6. As ST5/ST6 was respectively 4-hydroxy-FCF and 3-hydroxy-FCF, therefore, TP12 was identified as 2-hydroxy-FCF. The chemical structure and the fragment pathway of TP12 had been concluded and shown in Fig 4E.

The protonated molecular ion of TP9 (see Fig 4D, Table 2, m/z 426.1045, error -4.2 ppm), retention time 5.610 min, yielded three MS2 ions at m/z 307.0664(error, -8.8 ppm), m/z 264.0560 (error, 9.8 ppm), m/z 145.0169 (error, 4.1 ppm). As can be seen in Table 1, the fragment ion at m/z 248.0585, m/z 264.0540 was respectively the characteristic ions of M0, ST5, ST6, and the fragment ion at m/z 264.0564 is 15.9979 Da (O) higher than that of m/z 248.0585 (M0), also the MS2 ion at m/z 264.0564 was formed through neutral losing 162.0510 (C6H10O5). Thus TP9 was the glycosylation product of ST5/ST6. The product ion at m/z 307.0664 could lead to the MS3 ion at m/z 145.0175 by the neutral loss of 162.0489 (C6H10O5), and the fragment ion at m/z 145.0175 was 15.9961 Da (O) higher than the fragment ion at m/z 129.0214 of ST1. Thus, we conclude the hydroxylated position was located on pyridine ring of M0. Thus, TP9 was identified as 1-(2-chloro-6-((3, 4, 5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl) oxy) pyridin-4-yl)-3-phenylurea.The proposed chemical structure and the fragment pathway for TP9 were shown in Fig 4D.

TP13 (see Fig 4E, Table 2), m/z 283.0125(error, -8.1 ppm) lead to two main MS2 ions at m/z [M+H]+ m/z 154.9998 (error, -5.8 ppm), m/z 129.0208 (error, -4.7 ppm), which were the characteristic fragment ions of M0. Meanwhile, we found 3 molecular ions at m/z 283.0162, m/z 285.0132, m/z 287.0113 were present in relative intensities of about 9:6:1 in MS1 ions, which was the natural abundance ratio of 35Cl and 37Cl. Thus, we deduced that there were the two chlorine atoms in TP13. The fragment ion at m/z 154.9998 was generated by the neutral loss of m/z 128.0127 Da (C5H5ClN2). On the other hand, the fragment ion at m/z 129.0208 was generated by the neutral loss of m/z 153.9917 Da (C6H3ClN2O). TP13 was calculated as C11H8Cl2N4O by the Formula Predictor software. Thus, TP13 was identified as 1, 3-bis (2-chloropyridin-4-yl) urea. The chemical structure and the fragment pathway are shown in Fig 4E.

TP14 (see Fig 4F, Table 2) showed the protonated molecular ion [M+H]+ at m/z 291.0636, was 43.0051 Da higher than that of the molecular ion of M0 (m/z 248.0585) and the ratio of the relative intensities of the principal isotopes, A to A+2 was approximately 3:1, suggesting the presence of a chlorine atom (the natural abundance of 35Cl: 37Cl is 3:1). The neutral loss of 43.0051 Da was calculated as HNCO by the Formula Predictor software. The MS2 (m/z 137.0711, error, 1.5 ppm) was formed through neutral losing of m/z 153.9932 (C6H3ClN2O). Thus, we concluded the fragment ion at m/z 137.0711 was the combination of HNCO and aniline, and its combination position was located on amino of aniline. Further analysis showed the fragment ion at m/z 137.0711 was formed by the reaction of aniline and amino acid in kiwifruit sample. Thus, TP14 was identified as 1-carbamoyl-3-(2-chloropyridin-4-yl) -1-phenylurea. The chemical structure and the fragment pathway of TP14 are shown in Fig 4F.

TP15 (see Fig 4G, Table 2, m/z 213.1021, error, -0.5 ppm) was calculated as C13H12N2O by the Formula Predictor software according to the accurate mass measurement. As can be seen in Fig 4G, the molecular ion of TP15 showed the major product ions at m/z 94.0651 (error, 0 ppm). Thus, TP15 was identified as 1, 3-diphenylurea, and its chemical structures and fragment pattern were shown in Fig 4F.

TP16 (see Fig 4G, Table 2) was detected at the retention time of 7.086 min and gave a protonated molecule ion at m/z 262.0744 (error, 0.8 ppm), and there were two characteristic fragment ions at m/z 154.9999, m/z 129.0215 of M0. The protonated molecule at m/z 262.0744 was 14.0157 Da (CH2) higher than that of M0, and the product ion of m/z 108.0813 was 14.0162 Da (CH2) higher than that of phenylamine, and also the MS2 ion at m/z 108.0813 was generated through the neutral loss of C6H3ClN2O. Thus, we concluded the position of CH2 was located at the benzene ring, and it could be 1-(2-chloropyridin-4-yl)-3-(o-tolyl) urea or 1-(2-chloropyridin-4-yl)-3-(m-tolyl) urea or 1-(2-chloropyridin-4-yl)-3-(p-tolyl) urea. Its accurate structure need to be further identified by the reference standards. We tentatively concluded the proposed structure and the fragment pathway of TP16 were shown in Fig 4G.

TP17 (see Fig 4F, Table 2) showed the protonated molecular ion [M+H]+ at m/z 367.0980 (a 37Cl isotope signal of m/z 369.0927), and three main MS2 ions at m/z 248.0574 (error, -4.4 ppm), m/z 213.1036 (error, 6.6 ppm) and the fragment ion at m/z 213.1036 could lead to one MS3 product ions at m/z 94.0658. The products ions at m/z 248.0574, m/z 213.1036 were calculated as respectively C12H10ClN3O (M0), C13H12N2O (TP15) by Formula Predictor software. Thus, TP17 was a reaction product of M0 and TP15 and identified as 3-(2-chloropyridin-4-yl)-1-phenyl-1- (phenylcarbamoyl)urea. The chemical structures of TP17 and its fragment pattern were shown in Fig 4F.

Degradation and metabolism pathway of forchlorfenuron in kiwifruit

According to the deduced structure formulas of the 17 TPs (Fig 5), the degradation and metabolism pathway of FCF in kiwifruit were proposed and are shown in Fig 6. The present study showed that 17 TPs could be found and determined, several of which were discovered for the first time. These data indicate some metabolic pathways of FCF, such as, FCF was cleaved to TP1, TP2, TP4, its hydroxylated product is TP8, TP10, TP12, furthermore, its glycosylation product is TP3, TP6, TP9.

Fig 5

The proposed chemical structure of 17 TPs.

Fig 6

Proposed degradation and metabolism pathway of FCF in kiwifruit.

Conclusions

Total 17 TPs of FCF in postharvest kiwifruit were detected by LCMS-IT-TOF, and the main transformation pathways were hydroxylation, glycosylation, methylation, cleavage, oxidation, reduction, and so on. The experimental results indicate that LCMS-IT-TOF is powerful and effective tool for identification of plant growth regulation TPs.