Xiao Li1, Ying-Ying Chen2, Xiu-Mei Wang2, Kai Gao3, Yun-Zhou Gao4, Jian Cao1, Zhuo-Li Zhang5, Jing Lei1, Zheng-Yu Jin1, Yi-Ning Wang1. 1. Department of Radiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730, China. 2. Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua UniversityBeijing 100084, China. 3. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021, China. 4. Department of Pathology and Center for Experimental Animal Research, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005, China. 5. Department of Radiology, Feinberg School of Medicine, Northwestern UniversityChicago, IL 60611, USA.
Abstract
AIM: To investigate the survival of bone marrow mesenchymal stem cells (BMSCs) and the therapeutic effect for acute myocardial infarction (AMI) after co-transplantation with the functionalized self-assembling peptide nanofiber RAD/PRG or RAD/KLT. METHODS: AMI of balb/c mice was induced. Mice were randomly divided into four groups, and received treatment of phosphate buffered saline (PBS) (Group A), GFP/Fluc-BMSCs (Group B), GFP/Fluc-BMSCs + RAD/PRG (Group C), and GFP/Fluc-BMSCs + RAD/KLT (Group D), respectively. Bioluminescence imaging (BLI) was performed on day 1 (d-1), d-4, d-7, d-10, and d-13 after transplantation. Magnetic resonance imaging (MRI) was performed at baseline (d-4 before transplantation) and d-29 after treatment. Mice were euthanized on d-29 following treatment. Paraffin sections were obtained from the top, mid and bottom part of the infarcted region along the long-axis of the heart. Hematoxylin and eosin (HE) staining and immunohistochemical staining were performed. The infarct ratio micro-vascular density (MVD) was quantified. RESULTS: There was a significant higher of BLI signal intensity of BMSCs in Group C than that in Group B (d-4, 9713±320 vs. 8164±378, P=0.0008; d-7, 6489±241 vs. 5417±361, P=0.0026; d-10, 3768±255 vs. 0, P < 0.0001). The left ventricular ejection fraction (LVEF) via MRI examination was significantly improved in both Group C and Group D. Infarct ratio and MVD were significantly improved in both Group C and Group D. CONCLUSION: Our data highlights BMSCs combining functionalized self-assembling peptide nanofibers RAD/PRG or RAD/KLT as promising therapy for AMI.
AIM: To investigate the survival of bone marrow mesenchymal stem cells (BMSCs) and the therapeutic effect for acute myocardial infarction (AMI) after co-transplantation with the functionalized self-assembling peptide nanofiber RAD/PRG or RAD/KLT. METHODS: AMI of balb/c mice was induced. Mice were randomly divided into four groups, and received treatment of phosphate buffered saline (PBS) (Group A), GFP/Fluc-BMSCs (Group B), GFP/Fluc-BMSCs + RAD/PRG (Group C), and GFP/Fluc-BMSCs + RAD/KLT (Group D), respectively. Bioluminescence imaging (BLI) was performed on day 1 (d-1), d-4, d-7, d-10, and d-13 after transplantation. Magnetic resonance imaging (MRI) was performed at baseline (d-4 before transplantation) and d-29 after treatment. Mice were euthanized on d-29 following treatment. Paraffin sections were obtained from the top, mid and bottom part of the infarcted region along the long-axis of the heart. Hematoxylin and eosin (HE) staining and immunohistochemical staining were performed. The infarct ratio micro-vascular density (MVD) was quantified. RESULTS: There was a significant higher of BLI signal intensity of BMSCs in Group C than that in Group B (d-4, 9713±320 vs. 8164±378, P=0.0008; d-7, 6489±241 vs. 5417±361, P=0.0026; d-10, 3768±255 vs. 0, P < 0.0001). The left ventricular ejection fraction (LVEF) via MRI examination was significantly improved in both Group C and Group D. Infarct ratio and MVD were significantly improved in both Group C and Group D. CONCLUSION: Our data highlights BMSCs combining functionalized self-assembling peptide nanofibers RAD/PRG or RAD/KLT as promising therapy for AMI.
Authors: Yanyi Xu; Sourav Patnaik; Xiaolei Guo; Zhenqing Li; Wilson Lo; Ryan Butler; Andrew Claude; Zhenguo Liu; Ge Zhang; Jun Liao; Peter M Anderson; Jianjun Guan Journal: Acta Biomater Date: 2014-04-24 Impact factor: 8.947
Authors: Adam R Williams; Konstantinos E Hatzistergos; Benjamin Addicott; Fred McCall; Decio Carvalho; Viky Suncion; Azorides R Morales; Jose Da Silva; Mark A Sussman; Alan W Heldman; Joshua M Hare Journal: Circulation Date: 2012-12-05 Impact factor: 29.690
Authors: Martin Rodriguez-Porcel; Olivier Gheysens; Ramasamy Paulmurugan; Ian Y Chen; Karen M Peterson; Jürgen K Willmann; Joseph C Wu; Xiangyang Zhu; Lilach O Lerman; Sanjiv S Gambhir Journal: Mol Imaging Biol Date: 2009-12-15 Impact factor: 3.488
Authors: Philippe Menasché; Ottavio Alfieri; Stefan Janssens; William McKenna; Hermann Reichenspurner; Ludovic Trinquart; Jean-Thomas Vilquin; Jean-Pierre Marolleau; Barbara Seymour; Jérôme Larghero; Stephen Lake; Gilles Chatellier; Scott Solomon; Michel Desnos; Albert A Hagège Journal: Circulation Date: 2008-02-19 Impact factor: 29.690
Authors: Andrea S Carlini; Roberto Gaetani; Rebecca L Braden; Colin Luo; Karen L Christman; Nathan C Gianneschi Journal: Nat Commun Date: 2019-04-15 Impact factor: 14.919