Literature DB >> 2880792

A simple DNA diagnostic method for human genetic disorders.

D Rabin, N Dattagupta.   

Abstract

A fast, reliable, and simple technique for detecting point mutations in unfractionated human DNA has been developed. Oligonucleotide probes complementary to either sickle- or normal beta-globin DNA are labeled by primer extension and hybridized to DNA applied to nitrocellulose paper in a dot-blot format. A short hybridization time (about 1 h) and low probe concentration (about 1 nM) yield low background and high specificity. Double-blind trials show 100% agreement with restriction fragment length polymorphism (RFLP) analysis of DNA from normal, sickle, and heterozygous subjects.

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Year:  1987        PMID： 2880792     DOI： 10.1007/bf00591071

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  10 in total

1.  A general method for isolation of high molecular weight DNA from eukaryotes.

Authors:  N Blin; D W Stafford
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

2.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

3.  Detection of single base substitutions in total genomic DNA.

Authors:  R M Myers; N Lumelsky; L S Lerman; T Maniatis
Journal:  Nature       Date:  1985 Feb 7-13       Impact factor: 49.962

4.  Improved detection of the sickle mutation by DNA analysis: application to prenatal diagnosis.

Authors:  S H Orkin; P F Little; H H Kazazian; C D Boehm
Journal:  N Engl J Med       Date:  1982-07-01       Impact factor: 91.245

5.  A sensitive new prenatal test for sickle-cell anemia.

Authors:  J C Chang; Y W Kan
Journal:  N Engl J Med       Date:  1982-07-01       Impact factor: 91.245

6.  Direct identification of sickle cell anemia by blot hybridization.

Authors:  R F Geever; L B Wilson; F S Nallaseth; P F Milner; M Bittner; J T Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

7.  Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Authors:  J Brandsma; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

8.  Use of restriction endonucleases for mapping the allele for beta s-globin.

Authors:  J T Wilson; P F Milner; M E Summer; F S Nallaseth; H E Fadel; R H Reindollar; P G McDonough; L B Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

9.  Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.

Authors:  B J Conner; A A Reyes; C Morin; K Itakura; R L Teplitz; R B Wallace
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

10.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575
  10 in total
  1 in total

Review 1.  Automated mutation analysis.

Authors:  D Ravine
Journal:  J Inherit Metab Dis       Date:  1999-06       Impact factor: 4.982
  1 in total

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