Anne Becker1, Max Masthoff1, Jing Claussen2, Steven James Ford2, Wolfgang Roll3, Matthias Burg1, Peter J Barth4, Walter Heindel1, Michael Schäfers3,5, Michel Eisenblätter1,6, Moritz Wildgruber7. 1. Department of Clinical Radiology, University Hospital Muenster, Albert-Schweitzer-Campus 1, A16, 49149, Muenster, Germany. 2. iThera Medical, Munich, Germany. 3. Department of Nuclear Medicine, University Hospital Muenster, Muenster, Germany. 4. Gerhard-Domagk-Institute of Pathology, University Hospital Muenster, Muenster, Germany. 5. European Institute for Molecular Imaging - EIMI, University of Muenster, Muenster, Germany. 6. Divison of Imaging Sciences & Biomedical Engineering, King's College London, London, UK. 7. Department of Clinical Radiology, University Hospital Muenster, Albert-Schweitzer-Campus 1, A16, 49149, Muenster, Germany. moritz.wildgruber@ukmuenster.de.
Abstract
BACKGROUND AND AIM: Multispectral optoacoustic tomography (MSOT) represents a new in vivo imaging technique with high resolution (~250 μm) and tissue penetration (>1 cm) using the photoacoustic effect. While ultrasound contains anatomical information for lesion detection, MSOT provides functional information based on intrinsic tissue chromophores. We aimed to evaluate the feasibility of combined ultrasound/MSOT imaging of breast cancer in patients compared to healthy volunteers. METHODS: Imaging was performed using a handheld MSOT system for clinical use in healthy volunteers (n = 6) and representative patients with histologically confirmed invasive breast carcinoma (n = 5) and ductal carcinoma in situ (DCIS, n = 2). MSOT values for haemoglobin and oxygen saturation were assessed at 0.5, 1.0 and 1.5 cm depth and selected wavelengths between 700 and 850 nm. RESULTS: Reproducible signals were obtained in all wavelengths with consistent MSOT signals in superficial tissue in breasts of healthy individuals. In contrast, we found increased signals for haemoglobin in invasive carcinoma, suggesting a higher perfusion of the tumour and tumour environment. For DCIS, MSOT values showed only little variation compared to healthy tissue. CONCLUSIONS: This preliminary MSOT breast imaging study provided stable, reproducible data on tissue composition and physiological properties, potentially enabling differentiation of solid malignant and healthy tissue. KEY POINTS: • A handheld MSOT probe enables real-time molecular imaging of the breast. • MSOT of healthy controls provides a reproducible reference for pathology identification. • MSOT parameters allows for differentiation of invasive carcinoma and healthy tissue.
BACKGROUND AND AIM: Multispectral optoacoustic tomography (MSOT) represents a new in vivo imaging technique with high resolution (~250 μm) and tissue penetration (>1 cm) using the photoacoustic effect. While ultrasound contains anatomical information for lesion detection, MSOT provides functional information based on intrinsic tissue chromophores. We aimed to evaluate the feasibility of combined ultrasound/MSOT imaging of breast cancer in patients compared to healthy volunteers. METHODS: Imaging was performed using a handheld MSOT system for clinical use in healthy volunteers (n = 6) and representative patients with histologically confirmed invasive breast carcinoma (n = 5) and ductal carcinoma in situ (DCIS, n = 2). MSOT values for haemoglobin and oxygen saturation were assessed at 0.5, 1.0 and 1.5 cm depth and selected wavelengths between 700 and 850 nm. RESULTS: Reproducible signals were obtained in all wavelengths with consistent MSOT signals in superficial tissue in breasts of healthy individuals. In contrast, we found increased signals for haemoglobin in invasive carcinoma, suggesting a higher perfusion of the tumour and tumour environment. For DCIS, MSOT values showed only little variation compared to healthy tissue. CONCLUSIONS: This preliminary MSOT breast imaging study provided stable, reproducible data on tissue composition and physiological properties, potentially enabling differentiation of solid malignant and healthy tissue. KEY POINTS: • A handheld MSOT probe enables real-time molecular imaging of the breast. • MSOT of healthy controls provides a reproducible reference for pathology identification. • MSOT parameters allows for differentiation of invasive carcinoma and healthy tissue.
Entities:
Keywords:
Breast cancer; In vivo imaging; Multispectral optoacoustic tomography; Optoacoustic imaging; Ultrasound
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