| Literature DB >> 28777546 |
Yang Liu1, Lizeth Perez1, Adam D Gill1, Magi Mettry1, Lin Li1, Yinsheng Wang1, Richard J Hooley1, Wenwan Zhong1.
Abstract
Arrayed deep cavitands can be coupled to a fluorescence-based supramolecular tandem assay that allows site-selective in situ monitoring of post-translational modifications catalyzed by the lysine methyltransferase PRDM9 or the lysine demethylase JMJD2E. An arrayed sensor system containing only three cavitand components can detect the specific substrates of enzyme modification, in the presence of other histone peptides in the enzyme assay, enabling investigation of cross-reactivity over multiple methylation sites and interference from nonsubstrate peptides.Entities:
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Year: 2017 PMID： 28777546 PMCID： PMC5615822 DOI： 10.1021/jacs.7b05002
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419