D Lauritano1, C A Bignozzi2, D Pazzi2, F Cura3, F Carinci4. 1. Department of Translational Medicine and Surgery, Neuroscience Center of Milan, University of Milano Bicocca, Monza, Italy. 2. Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy. 3. Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy. 4. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
Abstract
BACKGROUND: Titanium is the gold standard for dental implants, since it has demonstrated excellent biocompatibility and osseointegration properties. The rate of osseointegration is however affected by the surface morphology and peri-implant infections may affect fixture and the long-term osseointegration outcome. Thus chemical composition of a coating at the implant-abutment junction (IAJ) surface is expected to play a key role in preventing bacterial infection. PURPOSE: In the present study a new antimicrobial polysiloxane coating functionalized with chlorexidine digluconate (PXT) has been tested in an in vitro model. MATERIALS AND METHODS: Twenty implants were coated in the internal chamber with PXT and twenty were used as controls. RESULTS AND CONCLUSIONS: Ten of the coated implants, preliminarily tested against Gram positive and negative bacteria and fungi, showed a complete inactivation of the microbial species after a 15 min contact. On the remaining ten treated implants a series of microbiological tests and PCR analysis, after contamination of the implant external medium, in which the implant have been immersed, with genetic modified Tannerella forsythia (TF) and Porphyromonas Gingivalis (PG), leads to the conclusion that the coating is capable of inactivating the microbial species penetrating the internal of the implant through the implant abutment junction.
BACKGROUND:Titanium is the gold standard for dental implants, since it has demonstrated excellent biocompatibility and osseointegration properties. The rate of osseointegration is however affected by the surface morphology and peri-implant infections may affect fixture and the long-term osseointegration outcome. Thus chemical composition of a coating at the implant-abutment junction (IAJ) surface is expected to play a key role in preventing bacterial infection. PURPOSE: In the present study a new antimicrobial polysiloxane coating functionalized with chlorexidine digluconate (PXT) has been tested in an in vitro model. MATERIALS AND METHODS: Twenty implants were coated in the internal chamber with PXT and twenty were used as controls. RESULTS AND CONCLUSIONS: Ten of the coated implants, preliminarily tested against Gram positive and negative bacteria and fungi, showed a complete inactivation of the microbial species after a 15 min contact. On the remaining ten treated implants a series of microbiological tests and PCR analysis, after contamination of the implant external medium, in which the implant have been immersed, with genetic modified Tannerella forsythia (TF) and Porphyromonas Gingivalis (PG), leads to the conclusion that the coating is capable of inactivating the microbial species penetrating the internal of the implant through the implant abutment junction.
Authors: Antonio R C Duarte; Paulo H O Rossetti; Leylha M N Rossetti; Sergio A Torres; Wellington C Bonachela Journal: J Periodontol Date: 2006-11 Impact factor: 6.993
Authors: V Candotto; D Lauritano; M Nardone; L Baggi; C Arcuri; R Gatto; R M Gaudio; F Spadari; F Carinci Journal: Oral Implantol (Rome) Date: 2017-11-30
Authors: Francesco Carinci; Dorina Lauritano; Carlo Alberto Bignozzi; Daniele Pazzi; Valentina Candotto; Paulo Santos de Oliveira; Antonio Scarano Journal: Int J Mol Sci Date: 2019-08-09 Impact factor: 5.923