Literature DB >> 2874482

Amplification of the gene for histidyl-tRNA synthetase in histidinol-resistant Chinese hamster ovary cells.

F W Tsui, I L Andrulis, H Murialdo, L Siminovitch.   

Abstract

Histidinol-resistant (HisOHR) mutants with up to a 30-fold increase in histidyl-tRNA synthetase activity have been isolated by stepwise adaptation of wild-type Chinese hamster ovary (CHO) cells to increasing amounts of histidinol in the medium. Immunoprecipitation of [35S]methionine-labeled cell lysates with antibodies to histidyl-tRNA synthetase showed increased synthesis of the enzyme in histidinol-resistant cells. The histidinol-resistant cell lines had an increase in translatable polyadenylated mRNA for histidyl-tRNA synthetase. A cDNA for CHO histidyl-tRNA synthetase has been cloned, using these histidyl-tRNA synthetase-overproducing mutants as the source of mRNA. Southern blot analysis of wild-type and histidinol-resistant cells with this cDNA showed that the histidyl-tRNA synthetase DNA bands were amplified in the resistant cells. These HisOHR cells owed their resistance to histidinol to amplification of the gene for histidyl-tRNA synthetase.

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Year:  1985        PMID： 2874482      PMCID： PMC366965          DOI： 10.1128/mcb.5.9.2381-2388.1985

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  23 in total

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3.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

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4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

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5.  Mutations in the structural genes of CHO cell histidyl-, valyl-, and leucyl-tRNA synthetases.

Authors:  C R Ashman
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6.  Biochemical characterization of a mutant asparaginyl-tRNA synthetase from Chinese hamster ovary cells.

Authors:  I L Andrulis; C S Chiang; S M Arfin; T A Miner; G W Hatfield
Journal:  J Biol Chem       Date:  1978-01-10       Impact factor: 5.157

7.  Selection by [3H] amino acids of CHO-cell mutants with altered leucyl- and asparagyl-transfer RNA synthetases.

Authors:  L H Thompson; C P Stanners; L Siminovitch
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8.  Six complementation classes of conditionally lethal protein synthesis mutants of CHO cells selected by 3H-amino acid.

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9.  Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.

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Journal:  J Immunol       Date:  1976-11       Impact factor: 5.422

10.  Evidence for structural gene alterations affecting aminoacyl-tRNA synthetases in CHO cell mutants and revertants.

Authors:  L H Thompson; D J Lofgren; G M Adair
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  5 in total

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5.  Isolation, structure and expression of mammalian genes for histidyl-tRNA synthetase.

Authors:  F W Tsui; L Siminovitch
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  5 in total

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