Nariman Moradi1, Reza Fadaei2, Reza Ahmadi1, Milad Hajimirza Mohammad1, Serveh Shahmohamadnejad3, Masoumeh Tavakoli-Yaraki1, Hassan Aghajani4, Soudabeh Fallah5. 1. Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. 2. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 3. Department of Biology, Faculty of Science, Urmia University, Urmia, Iran. 4. Interventional Cardiology Department, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: Aghajanih@tums.ac.ir. 5. Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; Research Center of Pediatric Infectious Disease, Rasool Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. Electronic address: Fallah.s@iums.ac.ir.
Abstract
BACKGROUND: Data concerning the association of serum levels of vitamin D and metalloproteinases and vitamin D receptor gene polymorphism with coronary artery disease (CAD) is not fully demonstrated. The present study aimed to evaluate the association of vitamin D receptor gene polymorphism, serum levels of 25(OH) vitamin D and metalloproteinase-9 (MMP-9) with CAD. METHODS: 104 patients with CAD and 69 Non-CAD subjects were included in current study. Vitamin D receptor genotypes were determined by PCR-RFLP method. The 25(OH) vitamin D and MMP-9 were determined by ELISA assay. RESULTS: There was a significant reduction of vitamin D in CAD patients (P=0.001). The metalloproteinase 9 levels of CAD patient was increased significantly compared with controls (P=0.001). A significant reverse correlation also was found between MMP-9 concentration and 25(OH) vitamin D levels of patients (r=-0.28, P<0.001). In addition, we identified that VDR gene FokI polymorphism was significantly associated with CAD. Furthermore, MMP-9 levels of CAD patients with ff genotype of FokI polymorphism was higher significantly than patients with FF and Ff genotypes. It has been also found that MMP-9 levels of CAD patients with ff genotype of FokI polymorphism was higher significantly than patients with FF and Ff genotypes. CONCLUSION: Our results indicated that 25(OH) vitamin D, MMP-9 levels and VDR gene FokI polymorphisms play a critical role in the development and progression of CAD and may contribute to susceptibility to CAD in Iranian populations.
BACKGROUND: Data concerning the association of serum levels of vitamin D and metalloproteinases and vitamin D receptor gene polymorphism with coronary artery disease (CAD) is not fully demonstrated. The present study aimed to evaluate the association of vitamin D receptor gene polymorphism, serum levels of 25(OH) vitamin D and metalloproteinase-9 (MMP-9) with CAD. METHODS: 104 patients with CAD and 69 Non-CAD subjects were included in current study. Vitamin D receptor genotypes were determined by PCR-RFLP method. The 25(OH) vitamin D and MMP-9 were determined by ELISA assay. RESULTS: There was a significant reduction of vitamin D in CAD patients (P=0.001). The metalloproteinase 9 levels of CAD patient was increased significantly compared with controls (P=0.001). A significant reverse correlation also was found between MMP-9 concentration and 25(OH) vitamin D levels of patients (r=-0.28, P<0.001). In addition, we identified that VDR gene FokI polymorphism was significantly associated with CAD. Furthermore, MMP-9 levels of CAD patients with ff genotype of FokI polymorphism was higher significantly than patients with FF and Ff genotypes. It has been also found that MMP-9 levels of CAD patients with ff genotype of FokI polymorphism was higher significantly than patients with FF and Ff genotypes. CONCLUSION: Our results indicated that 25(OH) vitamin D, MMP-9 levels and VDR gene FokI polymorphisms play a critical role in the development and progression of CAD and may contribute to susceptibility to CAD in Iranian populations.
Authors: Alaa Shafie; Ahmad El Askary; Mazen Almehmadi; Hatem H Allam; Lamiaa K Elsayyad; Asmaa F Hassan; Bader B Althobaiti; Amin Nadheef; Aisha H Alharthi; Amal F Gharib Journal: In Vivo Date: 2022 May-Jun Impact factor: 2.406