Le Van Chuong1,2, Virapong Prachayasittikul1, Chartchalerm Isarankura Na Ayudhya1, Ratana Lawung1. 1. Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. 2. Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Abstract
BACKGROUND: An increasing of prevalence and diversification of plasmid-mediated AmpC (pAmpC) has been emerged worldwide. The incidence of pAmpC resulted in increasing β-lactamase production and conferred resistance to almost all β-lactam antibiotics excluding carbapenems. The lack of standard method for pAmpC identification and classification exert a challenge in epidemiological surveillance and infection control practices. METHODS: A robust, single tube multiplex PCR has been developed to classify six different pAmpC groups including CIT (CMY-2 like, LAT and CFE), ECB (ACT, MIR), MOX & CMY-1 like, DHA, ACC, and FOX. The developed method was optimized and validated by testing of sensitivity and specificity. RESULTS: Developed method can detect crude extracted DNA template at nano-scale (2.5 ηg) and has high discriminatory power as compared to phenotypic and commercial genotypic method. CONCLUSION: The developed method can be utilized for tracking the changes of clinically important resistance patterns and further investigation of occurrence and distribution of plasmid-mediated AmpC types.
BACKGROUND: An increasing of prevalence and diversification of plasmid-mediated AmpC (pAmpC) has been emerged worldwide. The incidence of pAmpC resulted in increasing β-lactamase production and conferred resistance to almost all β-lactam antibiotics excluding carbapenems. The lack of standard method for pAmpC identification and classification exert a challenge in epidemiological surveillance and infection control practices. METHODS: A robust, single tube multiplex PCR has been developed to classify six different pAmpC groups including CIT (CMY-2 like, LAT and CFE), ECB (ACT, MIR), MOX & CMY-1 like, DHA, ACC, and FOX. The developed method was optimized and validated by testing of sensitivity and specificity. RESULTS: Developed method can detect crude extracted DNA template at nano-scale (2.5 ηg) and has high discriminatory power as compared to phenotypic and commercial genotypic method. CONCLUSION: The developed method can be utilized for tracking the changes of clinically important resistance patterns and further investigation of occurrence and distribution of plasmid-mediated AmpC types.
Authors: Keith S Kaye; Howard S Gold; Mitchell J Schwaber; Lata Venkataraman; Youlin Qi; Paola C De Girolami; Matthew H Samore; Greg Anderson; J Kamile Rasheed; Fred C Tenover Journal: Antimicrob Agents Chemother Date: 2004-05 Impact factor: 5.191
Authors: E Ascelijn Reuland; John P Hays; Denise M C de Jongh; Eman Abdelrehim; Ina Willemsen; Jan A J W Kluytmans; Paul H M Savelkoul; Christina M J E Vandenbroucke-Grauls; Nashwan al Naiemi Journal: PLoS One Date: 2014-03-18 Impact factor: 3.240