Microbial biotechnology addressing the plastic waste disaster.

Oceans are a major source of biodiversity, they provide livelihood, and regulate the global ecosystem by absorbing heat and CO2 . However, they are highly polluted with plastic waste. We are discussing here microbial biotechnology advances with the view to improve the start and the end of life of biodegradable polymers, which could contribute to the sustainable use of marine and coastal ecosystems (UN Sustainability development goal 14).

In 2015, global plastic production reached 322 million tonnes, with China accounting for 27.8% of world's plastic production, followed by the EU and USA contributing 18.5% each to world plastic production (Europe, 2016). The packaging segment makes up 39.9% of the plastic market. These mainly single‐use, disposable products greatly contribute to the convenience of modern life. However, their low recycling rates means we are producing large volumes of waste and the plastic waste fraction of municipal solid waste (MSW) is increasing. In the EU and the USA, 31% and 53%, respectively, of MSW including plastics are landfilled (EC, 2015; EPA, 2015).

While plastics pose many problems for terrestrial environments, plastic waste is also a major pollutant in the world's Oceans, resulting in death of wildlife (Rochman et al., 2013a,b; Wilcox et al., 2015). While the evidence for the presence of plastic in virtually all marine niche is overwhelming (Carson et al., 2011; Claessens et al., 2011; Frère et al., 2017; Kanhai et al., 2017), it is unknown how much plastic exactly is in the oceans and in what form (Cressey, 2016). Up to 12.7 million tonnes of plastic waste, generated in 2010 by 192 costal countries, ended up in the ocean (Jambeck et al., 2015). Data on microplastic concentrations and toxicity seem to be open for debate (Cressey, 2016). However, there is evidence that environmentally relevant concentrations of microplastic negatively affect sea life (Sussarellu et al., 2016). Over three billion people depend on marine and coastal biodiversity (UN, 2015): oceans provide food, medicines and other biobased products (EC, 2012). Furthermore, oceans are buffering the impacts of global warming by absorbing approximately 30% of the CO2 in the atmosphere (UN, 2015). Therefore, careful management of this essential global resource is of great importance for sustainability and it is recognized as one of the goals of the 2030 Agenda for sustainable development (UN, 2015).

An effective way to improve resource efficiency and reduce the environmental impact of plastics is the prevention of waste. The EU and EPA strategies for waste management include prevention, reuse, recycling, other recovery and disposal as the last resource (EC, 2013b; EPA, 2014). However, it is realistic to assume that post‐consumer plastics will end up in unmanaged environments. To reduce or prevent the negative impacts of post‐consumer plastic waste, society could replace conventional plastic materials with biodegradable counterparts. Biodegradable plastics can contribute to a more sustainable society using renewable resources, contribute to the reduction in CO2 emissions during production and offer new end‐of‐life management options that have a lower or no negative impact on the environment (EC, 2013a; Bioplastics, 2016). Biodegradable polymers are degradable in nature and include polylactic acid (PLA), thermoplastic starch (TPS), polyhydroxyalkanoate (PHA), polycaprolactone (PCL) and poly(butylene adipate‐co‐terephthalate) (PBAT). While PLA, TPS and PHA are also biobased, PCL and PBAT are fossil based. Thus, the origin of the polymer does not necessarily affect its end‐of‐life fate. Indeed, the biobased or natural origins of a polymer do not mean it is biodegradable. For example, technologies are emerging that can make polyethylene (PE), polyethylene terephthalate (PET) from biobased resources, but they are not biodegradable and thus their origin will not address the end‐of‐life pollution challenges.

PLA made up 5.1% of global bioplastic production in 2016, while PHA was represented by 1.6% of 4.16 million tonnes of globally produced bioplastic (Bioplastics, E., 2016). Even though PHAs have desirable properties such as elasticity, hydrophobicity, low oxygen permeability and biodegradability, they have not fully penetrated the plastic market (total production estimated at single figure thousands of tonne) due to uncompetitive pricing compared to fossil based plastics.

PLA and PHA are of a microbial origin, biobased and biodegradable and therefore address both the start and end of the plastic life cycle. PHAs are entirely a product of microbial metabolism, while PLA is produced through a combination of fermentation (to produce lactic acid) and chemistry to convert the lactic acid or lactide to PLA. PHAs are a family of intracellular polyesters that include polymers with very different physical properties (from highly crystalline and brittle to amorphous liquids), which opens up opportunities for different applications from packaging to medicine (Chen, 2009).

Researchers have attempted to address both the cost of production and waste management using different types of waste, including plastic waste, as a cheap feedstock for PHA production, for example polystyrene (Goff et al., 2007), polyethylene terephthalate (PET) (Kenny et al., 2012), waste glycerol (Cavalheiro et al., 2009), animal‐based waste streams (Titz et al., 2012), syngas obtained by municipal solid waste (MSW) pyrolysis (Revelles et al., 2016) as well as using low cost biomass (Cerrone et al., 2015; Walsh et al., 2015).

Of particular interest to the emerging circular economy is the upcycling of plastic waste into biodegradable plastic (Goff et al., 2007; Kenny et al., 2008, 2012; Wierckx et al., 2015). While conventional recycling technologies are available, there are several limitations, including cost and relatively low quality of the recycled polymers. Employing the microbial cell factory to convert plastic waste into high value product provides an alternative to conventional recycling. Due to extreme recalcitrance of plastics to microbial degradation, this biotechnological process currently employs pyrolysis to produce oils, which are subsequently fed to bacteria (Goff et al., 2007; Kenny et al., 2012). However, microbial hydrolases capable of modifying or degrading plastics have emerged recently as a potential technology for plastic biodepolymerization (Wei and Zimmermann, 2017) allowing for a completely biological recycling of plastics. These enzymes could be tailored using the synthetic biology toolbox and then integrated into a microbial chassis to design a custom microbial platform capable of converting plastic into biodegradable counterparts in a single cell (www.p4sb.eu).

The concept of a microbial platform relates also to the concept of minimal cell (Nikel et al., 2014). Desirable features of a minimized cell are efficient cell reproduction with minimal genetic drift, efficient control of transcription and translation, and predictable metabolic interactions. The deletion of the flagellar machinery, four prophages, two transposons and three components of DNA restriction‐modification systems in a PHA producer Pseudomonas putida KT2440 yielded a minimized cell which achieved higher specific growth rates and higher biomass, tolerated endogenous oxidative stress better, acquired and replicated exogenous DNA, and survived better in stationary phase (Martínez‐García et al., 2014). In addition, the bacterial morphology could be engineered to allow larger space for storage of PHA and convenient downstream processing (Jiang and Chen, 2016). Even though several targets have been identified for bacterial morphology modification, this concept of morphology engineering is still novel and requires development.

While PLA sales are growing year on year, the production cost can be reduced further. The microbial conversion of cheap substrates (Zhang and Vadlani, 2013; Muller et al., 2017) and waste (Panesar and Kaur, 2015; Pleissner et al., 2017) into lactic acid has been investigated. In parallel with efforts to improve the chemical synthesis of PLA (Dusselier et al., 2015), enzymatic polymerization of lactide is underway (Lassalle and Ferreira, 2008; Jeon et al., 2013). Furthermore, a synthetic pathway containing propionate‐CoA transferase from Clostridium propionicum and Pseudomonas sp. MBEL 6‐19 PHA polymerase was introduced into Escherichia coli, which allowed conversion of glucose into lactyl‐CoA and its polymerization into homopolymer PLA or into a heteropolymer poly(3‐hydroxybutyrate‐co‐lactate) (Yang et al., 2010). However, the production of the homopolymer was very poor giving only 0.5% of the cell dry weight as PLA, but it is a promising first step.

Given the additional challenge to improve the thermal and mechanical properties of new biobased and biodegradable polymers, copolymers have been generated (Yang et al., 2013). For example, a novel lactic acid containing terpolyester poly(lactate‐co‐3‐hydroxybutyrate‐co‐3‐hydroxypropionate) was produced by a recombinant E. coli (Ren et al., 2017). The engineered pathway contains 3‐hydroxypropionyl‐CoA synthesis pathway from glycerol, 3‐hydroxybutyryl‐CoA and lactyl‐CoA (LA‐CoA) pathways from glucose and an engineered PHA polymerase from P. stutzeri (Ren et al., 2017). Metabolically engineered E. coli is capable of converting renewable and sustainable resources, glucose and glycerol into the novel terpolyester. Furthermore, just by varying glucose‐to‐glycerol ratio composition of monomers in the terpolyester could be adjusted, which opens up the possibility to tailor the polymer properties.

The creation of composites of biopolymers can generate new materials with improved properties due to synergistic and additive benefits of the combination of polymers (Zhang et al., 1996; Broz et al., 2003; Yu et al., 2006). The compatibilization of polymers remains a major challenge, but nanotechnology is being studied to address this (Dufresne et al., 2013).

The advances in microbial biotechnology are creating exciting possibilities to design novel pathways to known biodegradable polymers, but also pathways to novel biodegradable polymers, which address the start and end of life of materials, for the benefit of consumers and the environment. While EU and US EPA place prevention at the top of waste management solutions, certain applications, that is fishing, would inevitably lead to plastic products reaching the ocean. Replacing conventional plastic with biodegradable counterparts should therefore be included into a wider concept of plastic waste management (Fig. 1).

Figure 1

An overview of possibilities created by implementation of biodegradable plastic. Biobased resources and/or waste are used as a feeding stock for the production of plastic, which can be biobased, biodegradable or both. The products made from plastic can be reused, recycled and in the case of biodegradable plastic, that is polyhydroxyalkanoate (PHA), polylactic acid (PLA), thermoplastic starch (TPS) biodegraded to provide new feed stocks for the microbial and/or chemical conversion into plastic, therefore closing the cycle. For applications that would inevitably lead to plastic products reaching the environment, implementation of biodegradable plastic could be used to reduce and prevent the accumulation of plastic waste.

Conflict of interest

None declared.