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Literature DB >> 2870814

Large inverted duplications are associated with gene amplification.

Abstract

Amplified DNA can be found in arrays of large repeated units, with each repeat unit containing a marker gene and surrounding DNA sequences. Amplified DNA sequences from established cell lines were assessed for the presence of repeat units in the form of inverted duplications. Inverted duplicated DNA was detected by virtue of its concentration-independent resistance to S1 hydrolysis after denaturation and rapid renaturation. Using this assay, inverted duplications were detected in amplified DNA (both DM and HSR configurations) containing the myc gene (16-50 copies/cell) in four human tumor cell lines and in amplified DNA containing the CAD gene (30-200 copies/cell) in three PALA-resistant BHK cell lines. The widespread association of inverted duplications with amplified DNA must bear on the amplification mechanism.

Entities: Disease Gene Species

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Year: 1986 PMID： 2870814 DOI： 10.1016/0092-8674(86)90328-4

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

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Cited

63 in total

1. Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system.

Authors: C T Lin; Y L Lyu; H Xiao; W H Lin; J Whang-Peng
Journal: Nucleic Acids Res Date: 2001-08-15 Impact factor: 16.971

2. Inverted repeats as genetic elements for promoting DNA inverted duplication: implications in gene amplification.

Authors: C T Lin; W H Lin; Y L Lyu; J Whang-Peng
Journal: Nucleic Acids Res Date: 2001-09-01 Impact factor: 16.971

3. Formation of large palindromic DNA by homologous recombination of short inverted repeat sequences in Saccharomyces cerevisiae.

Authors: David K Butler; David Gillespie; Brandi Steele
Journal: Genetics Date: 2002-07 Impact factor: 4.562

4. The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.

Authors: Bill Wickstead; Klaus Ersfeld; Keith Gull
Journal: Genome Res Date: 2004-06 Impact factor: 9.043

10. Hairpin structures are the primary amplification products: a novel mechanism for generation of inverted repeats during gene amplification.

Authors: S Cohen; D Hassin; S Karby; S Lavi
Journal: Mol Cell Biol Date: 1994-12 Impact factor: 4.272

Large inverted duplications are associated with gene amplification.

1. Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system.

2. Inverted repeats as genetic elements for promoting DNA inverted duplication: implications in gene amplification.

3. Formation of large palindromic DNA by homologous recombination of short inverted repeat sequences in Saccharomyces cerevisiae.

4. The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.

5. Origin of adeno-associated virus DNA replication is a target of carcinogen-inducible DNA amplification.

6. A mechanism of palindromic gene amplification in Saccharomyces cerevisiae.

7. Assessment of palindromes as platforms for DNA amplification in breast cancer.

8. Cloning and structural analysis of the snap-back DNA of Pharbitis nil.

9. De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes.

10. Hairpin structures are the primary amplification products: a novel mechanism for generation of inverted repeats during gene amplification.