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Literature DB >> 28707688

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).

Qinglin Wu¹, Benjamin A Fenton¹, Jessica L Wojtaszek¹, Pei Zhou¹.

Abstract

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

Entities: Chemical Disease Gene

Year: 2017 PMID： 28707688 PMCID： PMC5723528 DOI： 10.1039/c7cc05021f

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

20 in total

1. A 2D ¹³C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding.

Authors: Guillaume Bouvignies; Lewis E Kay
Journal: J Biomol NMR Date: 2012-06-12 Impact factor: 2.835

2. (13)CHD2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.

Authors: Enrico Rennella; Rui Huang; Algirdas Velyvis; Lewis E Kay
Journal: J Biomol NMR Date: 2015-08-14 Impact factor: 2.835

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).

1. A 2D ¹³C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding.

2. (13)CHD2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.

Review 3. New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy.

Review 4. An NMR perspective on enzyme dynamics.

5. Visualizing side chains of invisible protein conformers by solution NMR.

6. Probing slowly exchanging protein systems via ¹³Cα-CEST: monitoring folding of the Im7 protein.

7. Hsp70 biases the folding pathways of client proteins.

Review 8. Hierarchy of RNA functional dynamics.

9. Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain.

10. Drug design from the cryptic inhibitor envelope.

1. Probing excited conformational states of nucleic acids by nitrogen CEST NMR spectroscopy.