Amol Ramchandra Gadbail1, Minal S Chaudhary2, Sachin C Sarode3, Madhuri Gawande2, Sheetal Korde4, Satyajit Ashok Tekade5, Shailesh Gondivkar6, Alka Hande2, Rupesh Maladhari2. 1. Department of Dentistry, Indira Gandhi Government Medical College and Hospital, Nagpur, Maharashtra, India. 2. Department of Oral Pathology and Microbiology, Sharad Pawar Dental College & Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (M), Wardha, Maharashtra, India. 3. Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Pune, Maharashtra, India. 4. Department of Oral Pathology and Microbiology, Yerala Dental College and Hospital, Kharghar, Mumbai, India. 5. Department of Oral Pathology and Microbiology, Modern Dental College & Research Centre, Indore, Madhya Pradesh, India. 6. Department of Oral Medicine and Radiology, Government Dental College & Hospital, Nagpur, Maharashtra, India.
Abstract
BACKGROUND: The binary system of oral epithelial dysplasia (OED) has never been investigated with reference to the carcinogenesis-related biomarkers. Hence, Ki67, CD105, and α-SMA immune-expressions were studied in oral potentially malignant disorders (OPMDs) to assess their relationship with the binary OED grading system of World Health Organization. METHODS: The study was carried out on paraffin-embedded tissues of 30 normal oral mucosa (NOM) and 140 OPMD cases. OPMD cases were classified into two groups "no/questionable/hyperkeratosis/mild"=low-risk epithelial dysplasia (LRED) and "moderate or severe"=high-risk epithelial dysplasia (HRED). The immunohistochemistry was carried out to evaluate the expression of Ki67, CD 105, and α-SMA antigen. RESULTS: According to the binary grading system of WHO, 69 (49.28%) cases were LRED, while 71 (50.71%) case showed HRED. There was significant increase in Ki67 labeling index (LI) from NOM to LRED to HRED (P=.000). Similarly, mean vascular density (MVD) also increased significantly from NOM to LRED to HRED (P=.000). The α-SMA expression was significantly higher in HERD compared to LRED and NOM (P=.000). A positive correlation was noted among Ki67 LI, MVD, and α-SMA expressions in NOM, LRED, and HRED (P=.000). CONCLUSION: The expressions of ki67, CD105, and α-SMA markers compliment binary grading system of OED in OPMDS, thus justifying its use in clinical practice.
BACKGROUND: The binary system of oral epithelial dysplasia (OED) has never been investigated with reference to the carcinogenesis-related biomarkers. Hence, Ki67, CD105, and α-SMA immune-expressions were studied in oral potentially malignant disorders (OPMDs) to assess their relationship with the binary OED grading system of World Health Organization. METHODS: The study was carried out on paraffin-embedded tissues of 30 normal oral mucosa (NOM) and 140 OPMD cases. OPMD cases were classified into two groups "no/questionable/hyperkeratosis/mild"=low-risk epithelial dysplasia (LRED) and "moderate or severe"=high-risk epithelial dysplasia (HRED). The immunohistochemistry was carried out to evaluate the expression of Ki67, CD 105, and α-SMA antigen. RESULTS: According to the binary grading system of WHO, 69 (49.28%) cases were LRED, while 71 (50.71%) case showed HRED. There was significant increase in Ki67 labeling index (LI) from NOM to LRED to HRED (P=.000). Similarly, mean vascular density (MVD) also increased significantly from NOM to LRED to HRED (P=.000). The α-SMA expression was significantly higher in HERD compared to LRED and NOM (P=.000). A positive correlation was noted among Ki67 LI, MVD, and α-SMA expressions in NOM, LRED, and HRED (P=.000). CONCLUSION: The expressions of ki67, CD105, and α-SMA markers compliment binary grading system of OED in OPMDS, thus justifying its use in clinical practice.
Authors: Oana Mihaela Condurache Hritcu; Ana Emanuela Botez; Doinita Temelie Olinici; P Onofrei; Laura Stoica; V B Grecu; Paula Mihaela Toader; Laura Gheucă-Solovăstru; Elena Carmen Cotrutz Journal: Exp Ther Med Date: 2021-06-04 Impact factor: 2.447