Gordana Supic1,2, Katarina Zeljic3, Aleksandra Divac Rankov4, Ruzica Kozomara5,6, Aleksandra Nikolic4, Dragica Radojkovic4, Zvonko Magic5,7. 1. Faculty of Medicine, Military Medical Academy, University of Defense, Belgrade, Serbia. gogasupic@sezampro.rs. 2. Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia. gogasupic@sezampro.rs. 3. Faculty of Biology, University of Belgrade, Belgrade, Serbia. 4. Institute for Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia. 5. Faculty of Medicine, Military Medical Academy, University of Defense, Belgrade, Serbia. 6. Clinic for Maxillofacial Surgery, Military Medical Academy, Belgrade, Serbia. 7. Institute for Medical Research, Military Medical Academy, Crnotravska 17, Belgrade, 11002, Serbia.
Abstract
OBJECTIVES: Micro RNAs (miRNAs) have a major role in human cancerogenesis.The current study investigated the prognostic significance of miR-183 and miR-21 expression in tongue carcinoma patients. MATERIAL AND METHOD: For qPCR of miR-183 and miR-21 expression, total RNA isolated from 60 fresh-frozen tissue of tongue carcinomas was converted into cDNA by TaqMan MicroRNA Reverse Transcription Kit and quantified by TaqMan MicroRNAs Expression Assays. Fold changes in the miRNAs expression, normalized to RNU6B, were determined using 2-ΔΔCt method, and dichotomized into high and low according to cut-off values derived from ROC curve analysis. RESULTS: miR-183 emerged as promising discriminatory biomarker of poor outcome. Tissue over-expression of miR-183, observed in 68.3% of tongue carcinomas, was associated with clinical stage (p = 0.037), tumor size (p = 0.036), and high alcohol intake (p = 0.034).The patients with miR-183 over-expression had significantly shorter overall survival (p = 0.006) and a 5.666 times higher risk of poor outcome (p = 0.005), while miR-21 over-expression carried a tendency towards poorer survival (p = 0.073). However, multivariate analysis revealed that the recurrences were independent adverse prognostic factors, while miR-183 over-expression lost its significance. CONCLUSION: Our results suggests that over-expression of miR-183 in tumor tissue could be a potential marker of clinical stage and a poor survival of tongue carcinoma patients and may be associated with high alcohol consumption. CLINICAL RELEVANCE: Oncogenic miRNAs, such as the investigated miR-183 and miR-21, could be novel prognostic biomarkers of tumor progression and adverse clinical outcome in oral cancer, as well as novel therapeutic targets in cancer.
OBJECTIVES: Micro RNAs (miRNAs) have a major role in human cancerogenesis.The current study investigated the prognostic significance of miR-183 and miR-21 expression in tongue carcinomapatients. MATERIAL AND METHOD: For qPCR of miR-183 and miR-21 expression, total RNA isolated from 60 fresh-frozen tissue of tongue carcinomas was converted into cDNA by TaqMan MicroRNA Reverse Transcription Kit and quantified by TaqMan MicroRNAs Expression Assays. Fold changes in the miRNAs expression, normalized to RNU6B, were determined using 2-ΔΔCt method, and dichotomized into high and low according to cut-off values derived from ROC curve analysis. RESULTS:miR-183 emerged as promising discriminatory biomarker of poor outcome. Tissue over-expression of miR-183, observed in 68.3% of tongue carcinomas, was associated with clinical stage (p = 0.037), tumor size (p = 0.036), and high alcohol intake (p = 0.034).The patients with miR-183 over-expression had significantly shorter overall survival (p = 0.006) and a 5.666 times higher risk of poor outcome (p = 0.005), while miR-21 over-expression carried a tendency towards poorer survival (p = 0.073). However, multivariate analysis revealed that the recurrences were independent adverse prognostic factors, while miR-183 over-expression lost its significance. CONCLUSION: Our results suggests that over-expression of miR-183 in tumor tissue could be a potential marker of clinical stage and a poor survival of tongue carcinomapatients and may be associated with high alcohol consumption. CLINICAL RELEVANCE: Oncogenic miRNAs, such as the investigated miR-183 and miR-21, could be novel prognostic biomarkers of tumor progression and adverse clinical outcome in oral cancer, as well as novel therapeutic targets in cancer.
Authors: Deepak Abraham; Nicole Jackson; Justin S Gundara; JingTing Zhao; Anthony J Gill; Leigh Delbridge; Bruce G Robinson; Stan B Sidhu Journal: Clin Cancer Res Date: 2011-05-27 Impact factor: 12.531
Authors: Jin-Feng Tang; Zhong-Hua Yu; Tie Liu; Zi-Ying Lin; Ya-Hong Wang; La-Wei Yang; Hui-Juan He; Jun Cao; Hai-Li Huang; Gang Liu Journal: Asian Pac J Cancer Prev Date: 2014
Authors: Mario Dioguardi; Francesca Spirito; Diego Sovereto; Mario Alovisi; Giuseppe Troiano; Riccardo Aiuto; Daniele Garcovich; Vito Crincoli; Luigi Laino; Angela Pia Cazzolla; Giorgia Apollonia Caloro; Michele Di Cosola; Lorenzo Lo Muzio Journal: Int J Environ Res Public Health Date: 2022-03-14 Impact factor: 3.390