Literature DB >> 28580176

Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA.

B E Tarn1, K-J Sung1, H D Sikes1.   

Abstract

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

Entities:  

Year:  2016        PMID： 28580176      PMCID： PMC5450655          DOI： 10.1039/C6ME00073H

Source DB:  PubMed          Journal:  Mol Syst Des Eng


  25 in total

1.  Direct DNA methylation profiling using methyl binding domain proteins.

Authors:  Yinni Yu; Steve Blair; David Gillespie; Randy Jensen; David Myszka; Ahmed H Badran; Indraneel Ghosh; Alexander Chagovetz
Journal:  Anal Chem       Date:  2010-06-15       Impact factor: 6.986

2.  Chemical approach toward efficient DNA methylation analysis.

Authors:  Akimitsu Okamoto
Journal:  Org Biomol Chem       Date:  2008-11-06       Impact factor: 3.876

3.  Low-density DNA microarrays are versatile tools to screen for known mutations in hypertrophic cardiomyopathy.

Authors:  Stephan Waldmüller; Petra Freund; Simon Mauch; Roland Toder; Hans-Peter Vosberg
Journal:  Hum Mutat       Date:  2002-05       Impact factor: 4.878

4.  Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysis.

Authors:  Brandon W Heimer; Brooke E Tam; Hadley D Sikes
Journal:  Protein Eng Des Sel       Date:  2015-09-18       Impact factor: 1.650

5.  Design and validation of a microarray for detection, hemagglutinin subtyping, and pathotyping of avian influenza viruses.

Authors:  Astrid Gall; Bernd Hoffmann; Timm Harder; Christian Grund; Dirk Höper; Martin Beer
Journal:  J Clin Microbiol       Date:  2008-12-03       Impact factor: 5.948

6.  Hypermethylation of the hMLH1 promoter in colon cancer with microsatellite instability.

Authors:  J M Cunningham; E R Christensen; D J Tester; C Y Kim; P C Roche; L J Burgart; S N Thibodeau
Journal:  Cancer Res       Date:  1998-08-01       Impact factor: 12.701

7.  Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Authors:  J G Herman; J R Graff; S Myöhänen; B D Nelkin; S B Baylin
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-03       Impact factor: 11.205

8.  Multitarget stool DNA testing for colorectal-cancer screening.

Authors:  Thomas F Imperiale; David F Ransohoff; Steven H Itzkowitz; Theodore R Levin; Philip Lavin; Graham P Lidgard; David A Ahlquist; Barry M Berger
Journal:  N Engl J Med       Date:  2014-03-19       Impact factor: 91.245

Review 9.  DNA methylation profiling in the clinic: applications and challenges.

Authors:  Holger Heyn; Manel Esteller
Journal:  Nat Rev Genet       Date:  2012-09-04       Impact factor: 53.242

10.  5-halogenated pyrimidine lesions within a CpG sequence context mimic 5-methylcytosine by enhancing the binding of the methyl-CpG-binding domain of methyl-CpG-binding protein 2 (MeCP2).

Authors:  Victoria Valinluck; Pingfang Liu; Joseph I Kang; Artur Burdzy; Lawrence C Sowers
Journal:  Nucleic Acids Res       Date:  2005-05-25       Impact factor: 16.971
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  2 in total

1.  An examination of critical parameters in hybridization-based epigenotyping using magnetic microparticles.

Authors:  Brooke E Tam; Yining Hao; Hadley D Sikes
Journal:  Biotechnol Prog       Date:  2018-07-27

2.  Evolved DNA Duplex Readers for Strand-Asymmetrically Modified 5-Hydroxymethylcytosine/5-Methylcytosine CpG Dyads.

Authors:  Benjamin C Buchmuller; Jessica Dröden; Himanshu Singh; Shubhendu Palei; Malte Drescher; Rasmus Linser; Daniel Summerer
Journal:  J Am Chem Soc       Date:  2022-02-14       Impact factor: 15.419
  2 in total

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