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Literature DB >> 285316

An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2.

R G Downing, C J Duggleby, R Villems, P Broda.

Abstract

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid.

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Year: 1979 PMID： 285316 DOI： 10.1007/bf00267938

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

5 in total

1. Two modes of loss of the Tol function from Pseudomonas putida mt-2.

Authors: S A Bayley; C J Duggleby; M J Worsey; P A Williams; K G Hardy; P Broda
Journal: Mol Gen Genet Date: 1977-07-20

2. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

Authors: J B Hansen; R H Olsen
Journal: J Bacteriol Date: 1978-07 Impact factor: 3.490

3. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

Authors: K Murray
Journal: Biochem J Date: 1973-03 Impact factor: 3.857

4. Restriction endonuclease mapping of DNA using in situ digestion in two-dimensional gels.

Authors: R Villems; C J Duggleby; P Broda
Journal: FEBS Lett Date: 1978-05-15 Impact factor: 4.124

5. Molecular sizes and relationships of TOL plasmids in Pseudomonas.

Authors: C J Duggleby; S A Bayley; M J Worsey; P A Williams; P Broda
Journal: J Bacteriol Date: 1977-06 Impact factor: 3.490

5 in total

9 in total

An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2.

1. Two modes of loss of the Tol function from Pseudomonas putida mt-2.

2. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

3. Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

4. Restriction endonuclease mapping of DNA using in situ digestion in two-dimensional gels.

5. Molecular sizes and relationships of TOL plasmids in Pseudomonas.

1. A cleavage map of the TOL plasmid of Pseudomonas putida mt-2.

2. Plasmid-determined silver resistance in Pseudomonas stutzeri isolated from a silver mine.

3. Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants.

4. Physical mapping of TOL plasmids pWWO and pND2 and various R plasmid-TOL derivatives from Pseudomonas spp.

5. Excision of the 40kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats.

6. A third transposable element, ISPpu12, from the toluene-xylene catabolic plasmid pWW0 of Pseudomonas putida mt-2.

7. Excision and integration of degradative pathway genes from TOL plasmid pWW0.

8. TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: enzyme regulation and DNA structure.

9. Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0.