| Literature DB >> 231727 |
Abstract
A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.Entities:
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Year: 1979 PMID: 231727 DOI: 10.1007/bf00267270
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925