Literature DB >> 2851534

Enhancement of the apparent cleavage specificities of restriction endonucleases: applications to megabase mapping of chromosomes.

M McClelland1, M Nelson.   

Abstract

We have described how DNA methylases may be used to enhance the apparent specificities of restriction endonucleases [9-11, 17, 18] to generate DNA fragments averaging 6000 to 270,000,000 base pairs on random DNA. Taking into account the non-random arrangement of natural DNA, we have predicted the rarity of certain recognition sequences in the genomes of several species.

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Substances：

Year:  1987        PMID： 2851534

Source DB:  PubMed          Journal:  Gene Amplif Anal        ISSN： 0275-2778


  7 in total

1.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

2.  Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

3.  Enzymatic cleavage of a bacterial genome at a 10-base-pair recognition site.

Authors:  M D Weil; M McClelland
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

4.  Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

Authors:  M McClelland; M Nelson; E Raschke
Journal:  Nucleic Acids Res       Date:  1994-09       Impact factor: 16.971

5.  Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage.

Authors:  Y Patel; E Van Cott; G G Wilson; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

6.  Enzymatic cleavage of a bacterial chromosome at a transposon-inserted rare site.

Authors:  J Hanish; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

7.  Dissection of the Salmonella typhimurium genome by use of a Tn5 derivative carrying rare restriction sites.

Authors:  K K Wong; M McClelland
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490
  7 in total

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