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Literature DB >> 2158082

Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage.

Y Patel¹, E Van Cott, G G Wilson, M McClelland.

Abstract

The DNA methylase M.Xbal was isolated from an E. coli recombinant clone. We deduce that the enzyme methylates at the sequence 5'-TCTAGm6A-3'. In combination with the methylation-dependent restriction endonuclease, DpnI (5'-Gm6A/TC-3'), DNA cleavage occurs at the sequence 5'-TCTAGA/TCTAGA-3'. This twelve-base-pair site should occur once every 16,000,000 base pairs in a random sequence of DNA. The exceptional rarity of the M.XbaI/DpnI sequence makes it an ideal candidate for transpositional integration of a unique cleavage site into bacterial genomes. Retrotransposition into mammalian genomes is also an attractive possibility.

Entities: Chemical Species

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Year: 1990 PMID： 2158082 PMCID： PMC330532 DOI： 10.1093/nar/18.6.1603

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

23 in total

1. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids.

Authors: M N SWARTZ; T A TRAUTNER; A KORNBERG
Journal: J Biol Chem Date: 1962-06 Impact factor: 5.157

2. Restriction endonucleases for pulsed field mapping of bacterial genomes.

Authors: M McClelland; R Jones; Y Patel; M Nelson
Journal: Nucleic Acids Res Date: 1987-08-11 Impact factor: 16.971

3. NotI and SfiI: restriction endonucleases with octanucleotide recognition sequences.

Authors: B Q Qiang; I Schildkraut
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

4. Site-specific cleavage of DNA at 8- and 10-base-pair sequences.

Authors: M McClelland; L G Kessler; M Bittner
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

5. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.

Authors: C Waalwijk; R A Flavell
Journal: Nucleic Acids Res Date: 1978-12 Impact factor: 16.971

6. A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.

Authors: S Lacks; B Greenberg
Journal: J Biol Chem Date: 1975-06-10 Impact factor: 5.157

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

8. The effect of codon usage on the oligonucleotide composition of the E. coli genome and identification of over- and underrepresented sequences by Markov chain analysis.

Authors: G J Phillips; J Arnold; R Ivarie
Journal: Nucleic Acids Res Date: 1987-03-25 Impact factor: 16.971

Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage.

1. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids.

2. Restriction endonucleases for pulsed field mapping of bacterial genomes.

3. NotI and SfiI: restriction endonucleases with octanucleotide recognition sequences.

4. Site-specific cleavage of DNA at 8- and 10-base-pair sequences.

5. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.

6. A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

8. The effect of codon usage on the oligonucleotide composition of the E. coli genome and identification of over- and underrepresented sequences by Markov chain analysis.

9. DNA methylation and the frequency of CpG in animal DNA.

1. A physical map of the Salmonella typhimurium LT2 genome made by using XbaI analysis.

2. Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-Sce I.

3. Enzymatic cleavage of a bacterial chromosome at a transposon-inserted rare site.

4. Statistical analyses of counts and distributions of restriction sites in DNA sequences.