S Mateo1, F Vidal2, M Parriego3, I Rodríguez4, V Montalvo3, A Veiga3,5, M Boada3. 1. Reproductive Medicine Service, Department of Obstetrics, Gynecology and Reproduction, Women's Health Dexeus, Gran Via Carles III, 71-75, 08028, Barcelona, Spain. silmat@dexeus.com. 2. Cell Biology Unit, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. 3. Reproductive Medicine Service, Department of Obstetrics, Gynecology and Reproduction, Women's Health Dexeus, Gran Via Carles III, 71-75, 08028, Barcelona, Spain. 4. Unit of Biostatistics, Department of Obstetrics, Gynecology and Reproduction, Women's Health Dexeus, Gran Via Carles III, 71-75, 08028, Barcelona, Spain. 5. Stem Cell Bank, Centre for Regenerative Medicine, Dr. Aiguader, 88, 08003, Barcelona, Spain.
Abstract
PURPOSE: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. METHODS: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization. RESULTS: Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child. CONCLUSIONS: These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.
PURPOSE: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. METHODS: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization. RESULTS: Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at day 5 was 3.4% (3/88). Only 2.3% (2/88) euploid blastocysts were obtained. In two couples and after counseling and patient agreement, the transfer of a euploid blastocyst from a 1PN 2PB ICSI zygote was performed resulting in the birth of a healthy child. CONCLUSIONS: These results open the possibility to consider embryos coming from 1PN 2PB ICSI zygotes for transfer when no other embryos from 2PN 2PB ICSI zygotes are available and if a PGS diagnosis of euploidy is obtained. Confirmation of biparental inheritance is strongly recommended.
Authors: A Mertzanidou; L Wilton; J Cheng; C Spits; E Vanneste; Y Moreau; J R Vermeesch; K Sermon Journal: Hum Reprod Date: 2012-10-09 Impact factor: 6.918
Authors: Lorena Rodrigo; Vanessa Peinado; Emilia Mateu; José Remohí; Antonio Pellicer; Carlos Simón; Manuel Gil-Salom; Carmen Rubio Journal: Fertil Steril Date: 2009-07-15 Impact factor: 7.329
Authors: D S Johnson; C Cinnioglu; R Ross; A Filby; G Gemelos; M Hill; A Ryan; D Smotrich; M Rabinowitz; M J Murray Journal: Mol Hum Reprod Date: 2010-07-19 Impact factor: 4.025
Authors: Zhihong Yang; Jiaen Liu; Gary S Collins; Shala A Salem; Xiaohong Liu; Sarah S Lyle; Alison C Peck; E Scott Sills; Rifaat D Salem Journal: Mol Cytogenet Date: 2012-05-02 Impact factor: 2.009