Eri Araki1, Fumiaki Itoi2, Hiroyuki Honnma3, Yukiko Asano4, Hisanori Oguri4, Kazuyo Nishikawa1. 1. Department of Infertility, Green Bell Clinic, Kitamachi 2-160, Toyota, Aichi, 471-0027, Japan. 2. Department of Infertility, Green Bell Clinic, Kitamachi 2-160, Toyota, Aichi, 471-0027, Japan. fumiaki-itoi@kishokai.or.jp. 3. Sapporo ART Clinic, Kita7jonishi 4-1-2, Kita-ku, Sapporo, Hokkaido, 060-0807, Japan. 4. Department of Infertility, Royal Bell Clinic, Midori-ku, Nagoya, Aichi, 458-0801, Japan.
Abstract
PURPOSE: In this study, we examined the correlation between pronucleus size and the potential for human single pronucleus (1PN) zygotes to develop into blastocysts after IVF and ICSI. METHODS: This study included 112 patients who underwent a total of 112 cycles of IVF/ICSI. To evaluate embryo development, 1PN zygotes were compared with 2PN zygotes in the same IVF/ICSI cycle (control cycles) using time-lapse live embryo imaging. To assess the potential for blastocyst formation, cutoff values for pronuclear area and diameter were established through receiver operating characteristic curve analysis, after which 1PN zygotes were classified based on those cutoff values. RESULTS: Among 1PN zygotes cultured to day 5/6, the rate of embryo development was significantly lower than from 2PN zygotes. However, the rates of blastocyst formation and good quality blastocysts from 1PN zygotes with large pronuclear areas (≥ 710 μm2) or diameters (≥ 31 μm) were significantly higher than from 1PN zygotes with smaller pronuclear areas (≤ 509, 510-609, and 610-709 μm2) or diameters (≤ 24, 25-27,and 28-30 μm) (P < 0.01). Moreover, the results for 1PN zygotes with large pronuclei were similar to those for 2PN zygotes. CONCLUSIONS: The developmental potential of 1PN zygotes with large pronuclear areas (≥ 710 μm2) or diameters (31 μm) appears to be similar to that of 2PN zygotes, and measurement of pronuclear area or diameter in 1PN zygotes is a simple, potentially useful, clinical method.
PURPOSE: In this study, we examined the correlation between pronucleus size and the potential for human single pronucleus (1PN) zygotes to develop into blastocysts after IVF and ICSI. METHODS: This study included 112 patients who underwent a total of 112 cycles of IVF/ICSI. To evaluate embryo development, 1PN zygotes were compared with 2PN zygotes in the same IVF/ICSI cycle (control cycles) using time-lapse live embryo imaging. To assess the potential for blastocyst formation, cutoff values for pronuclear area and diameter were established through receiver operating characteristic curve analysis, after which 1PN zygotes were classified based on those cutoff values. RESULTS: Among 1PN zygotes cultured to day 5/6, the rate of embryo development was significantly lower than from 2PN zygotes. However, the rates of blastocyst formation and good quality blastocysts from 1PN zygotes with large pronuclear areas (≥ 710 μm2) or diameters (≥ 31 μm) were significantly higher than from 1PN zygotes with smaller pronuclear areas (≤ 509, 510-609, and 610-709 μm2) or diameters (≤ 24, 25-27,and 28-30 μm) (P < 0.01). Moreover, the results for 1PN zygotes with large pronuclei were similar to those for 2PN zygotes. CONCLUSIONS: The developmental potential of 1PN zygotes with large pronuclear areas (≥ 710 μm2) or diameters (31 μm) appears to be similar to that of 2PN zygotes, and measurement of pronuclear area or diameter in 1PN zygotes is a simple, potentially useful, clinical method.
Entities:
Keywords:
Blastocyst culture; ICSI; IVF; Pronucleus size; Single pronucleus zygotes; Time-lapse live embryo imaging
Authors: Rowena Smith; Sue J Pickering; Anna Kopakaki; K J Thong; Richard A Anderson; Chih-Jen Lin Journal: Reproduction Date: 2021-05-10 Impact factor: 3.906