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Literature DB >> 2849114

Construction of a clonable, infectious, and tumorigenic mouse mammary tumor virus provirus and a derivative genetic vector.

Abstract

Molecular genetic studies of mouse mammary tumor virus (MMTV) have been hampered by the difficulty of cloning proviruses of milk-borne strains because of inhibitory sequences located in the gag gene. To surmount this barrier we have constructed a hybrid MMTV provirus composed of clonable 5' sequences (encompassing gag) from an endogenous MMTV provirus of C3H mice (Mtv-1) and 3' sequences from the milk-borne strain of MMTV in C3H mice, MMTV(C3H). Virions produced from XC cells transfected with this hybrid provirus are infectious in cell culture and tumorigenic in BALB/cJ mice. A vector derived from this provirus, containing the neomycin phosphotransferase gene (neo) and origins of replication from simian virus 40 and pBR322, is capable of transferring G418 resistance by virus infection in cell culture when supplied with viral proteins from either MMTV(C3H) or the hybrid MMTV. Expression of both hybrid and vector proviruses is inducible by dexamethasone in infected cells.

Entities: Chemical Disease Gene Species

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Year: 1988 PMID： 2849114 PMCID： PMC282828 DOI： 10.1073/pnas.85.24.9655

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. Mammary tumor virus DNA contains sequences required for its hormone-regulated transcription.

Authors: D S Ucker; S R Ross; K R Yamamoto
Journal: Cell Date: 1981-12 Impact factor: 41.582

2. In vitro synthesis of polypeptides encoded by the long terminal repeat region of mouse mammary tumour virus DNA.

Authors: C Dickson; R Smith; G Peters
Journal: Nature Date: 1981-06-11 Impact factor: 49.962

3. Nucleotide sequences at host-proviral junctions for mouse mammary tumour virus.

Authors: J E Majors; H E Varmus
Journal: Nature Date: 1981-01-22 Impact factor: 49.962

4. Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones.

Authors: E Buetti; H Diggelmann
Journal: Cell Date: 1981-02 Impact factor: 41.582

5. Characterization of endogenous and exogenous mouse mammary tumor virus proviral DNA with site-specific molecular clones.

Authors: B Groner; E Buetti; H Diggelmann; N E Hynes
Journal: J Virol Date: 1980-12 Impact factor: 5.103

6. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection.

Authors: P Andersson; M P Goldfarb; R A Weinberg
Journal: Cell Date: 1979-01 Impact factor: 41.582

7. Demonstration of the absence of infectious Rous virus in rat tumour XC, whose structurally intact cells produce Rous sarcoma when transferred to chicks.

Authors: J SVOBODA; P CHYLE; D SIMKOVIC; I HILGERT
Journal: Folia Biol (Praha) Date: 1963-04 Impact factor: 0.906

8. Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.

Authors: N E Hynes; N Kennedy; U Rahmsdorf; B Groner
Journal: Proc Natl Acad Sci U S A Date: 1981-04 Impact factor: 11.205

9. Regulatory and coding potential of the mouse mammary tumor virus long terminal redundancy.

Authors: L A Donehower; A L Huang; G L Hager
Journal: J Virol Date: 1981-01 Impact factor: 5.103

10. Purification and translation of murine mammary tumor virus mRNA's.

Authors: J P Dudley; H E Varmus
Journal: J Virol Date: 1981-07 Impact factor: 5.103

55 in total

1. Genes involved in breast cancer progression: analysis of global changes in gene expression or retroviral tagging?

Authors: Emmett V Schmidt
Journal: Am J Pathol Date: 2002-12 Impact factor: 4.307

2. The mouse mammary tumor virus transcription enhancers for hematopoietic progenitor and mammary gland cells share functional elements.

Authors: F U Reuss; J M Coffin
Journal: J Virol Date: 2000-09 Impact factor: 5.103

3. Both T and B cells shed infectious mouse mammary tumor virus.

Authors: J L Dzuris; T V Golovkina; S R Ross
Journal: J Virol Date: 1997-08 Impact factor: 5.103

4. An immunoreceptor tyrosine activation motif in the mouse mammary tumor virus envelope protein plays a role in virus-induced mammary tumors.

Authors: Susan R Ross; John W Schmidt; Elad Katz; Laura Cappelli; Stacy Hultine; Phyllis Gimotty; John G Monroe
Journal: J Virol Date: 2006-09 Impact factor: 5.103

5. Cellular and viral components that mediate glucocorticoid-regulated processing of retroviral envelope proteins.

Authors: M R Stallcup; J L Corey; R M Bedgood
Journal: Cell Biophys Date: 1991 Oct-Dec

6. A PLPPV sequence in the p8 region of Gag provides late domain function for mouse mammary tumor virus.

Authors: Lori V Coren; Kunio Nagashima; David E Ott
Journal: Virology Date: 2019-07-19 Impact factor: 3.616

7. Coexpression of exogenous and endogenous mouse mammary tumor virus RNA in vivo results in viral recombination and broadens the virus host range.

Authors: T V Golovkina; A B Jaffe; S R Ross
Journal: J Virol Date: 1994-08 Impact factor: 5.103