| Literature DB >> 31357166 |
Lori V Coren1, Kunio Nagashima2, David E Ott3.
Abstract
The late (L) domain sequence used by mouse mammary tumor virus (MMTV) remains undefined. Similar to other L domain-containing proteins, MMTV p8 and p14NC proteins are monoubiquitinated, suggesting L domain function. Site-directed mutagenesis of p8, PLPPV, and p14NC, PLPPL, sequences in MMTV Gag revealed a requirement only for the PLPPV sequence in virion release in a position-dependent manner. Electron microscopy of a defective Gag mutant confirmed an L domain budding defect morphology. The equine infectious anemia virus (EIAV) YPDL core L domain sequence and PLPPV provided L domain function in reciprocal MMTV and EIAV Gag exchange mutants, respectively. Alanine scanning of the PLPPV sequence revealed a strict requirement for the valine residue but only minor requirements for any one of the other residues. Thus, PLPPV provides MMTV L domain function, representing a fourth type of retroviral L domain that enables MMTV Gag proteins to co-opt cellular budding pathways for release.Entities:
Keywords: Budding; Gag; Gag assembly; Late domain; MMTV; Mouse mammary tumor virus; Retrovirus
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Year: 2019 PMID： 31357166 PMCID： PMC6952571 DOI： 10.1016/j.virol.2019.07.015
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616