| Literature DB >> 28474797 |
Samar Hodeib1,2, Saurabh Raj1,2, Maria Manosas3,4, Weiting Zhang1,2, Debjani Bagchi1,2, Bertrand Ducos1,2, Francesca Fiorini5, Joanne Kanaan2, Hervé Le Hir2, Jean-François Allemand1,2, David Bensimon1,2,6, Vincent Croquette1,2.
Abstract
Helicases are a broad family of enzymes that separate nucleic acid double strand structures (DNA/DNA, DNA/RNA, or RNA/RNA) and thus are essential to DNA replication and the maintenance of nucleic acid integrity. We review the picture that has emerged from single molecule studies of the mechanisms of DNA and RNA helicases and their interactions with other proteins. Many features have been uncovered by these studies that were obscured by bulk studies, such as DNA strands switching, mechanical (rather than biochemical) coupling between helicases and polymerases, helicase-induced re-hybridization and stalled fork rescue.Entities:
Keywords: DNA unwinding; Holliday junction migration; active unwinding; ds-DNA fork; fork regression; helicase/polymerase coupling; helicases; magnetic traps; polymerase; primase; primosome; replisome; strand annealing
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Year: 2017 PMID： 28474797 PMCID： PMC5477542 DOI： 10.1002/pro.3187
Source DB: PubMed Journal: Protein Sci ISSN： 0961-8368 Impact factor: 6.725