| Literature DB >> 28473638 |
Peter See1, Charles-Antoine Dutertre1,2, Jinmiao Chen1, Patrick Günther3, Naomi McGovern1, Sergio Erdal Irac2, Merry Gunawan4, Marc Beyer3,5, Kristian Händler3, Kaibo Duan1, Hermi Rizal Bin Sumatoh1, Nicolas Ruffin6, Mabel Jouve6, Ester Gea-Mallorquí6, Raoul C M Hennekam7, Tony Lim8, Chan Chung Yip9, Ming Wen2, Benoit Malleret1,10, Ivy Low1, Nurhidaya Binte Shadan1, Charlene Foong Shu Fen11, Alicia Tay1, Josephine Lum1, Francesca Zolezzi1, Anis Larbi1, Michael Poidinger1, Jerry K Y Chan1,12,13,14, Qingfeng Chen15, Laurent Rénia1, Muzlifah Haniffa4, Philippe Benaroch6, Andreas Schlitzer1,16, Joachim L Schultze3,5, Evan W Newell1, Florent Ginhoux17.
Abstract
Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.Entities:
Mesh：
Year: 2017 PMID： 28473638 PMCID： PMC7611082 DOI： 10.1126/science.aag3009
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728