Literature DB >> 2846272

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

A L Schwartz1, A Ciechanover, R A Brandt, H J Geuze.   

Abstract

Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recognize only unconjugated ubiquitin. Ubiquitin is found within the cytoplasm, nucleus, the microvilli, autophagic vacuoles and lysosomes.

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Year:  1988        PMID： 2846272      PMCID： PMC454677          DOI： 10.1002/j.1460-2075.1988.tb03158.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  28 in total

1.  Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.

Authors:  G Goldstein; M Scheid; U Hammerling; D H Schlesinger; H D Niall; E A Boyse
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205

2.  Specificity of binding of NH2-terminal residue of proteins to ubiquitin-protein ligase. Use of amino acid derivatives to characterize specific binding sites.

Authors:  Y Reiss; D Kaim; A Hershko
Journal:  J Biol Chem       Date:  1988-02-25       Impact factor: 5.157

3.  Metabolism of ubiquitinated histones.

Authors:  R S Wu; K W Kohn; W M Bonner
Journal:  J Biol Chem       Date:  1981-06-10       Impact factor: 5.157

4.  Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins.

Authors:  A Hershko; E Eytan; A Ciechanover; A L Haas
Journal:  J Biol Chem       Date:  1982-12-10       Impact factor: 5.157

5.  Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds.

Authors:  S Matsui; A A Sandberg; S Negoro; B K Seon; G Goldstein
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

6.  Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.

Authors:  A Ciechanover; S Elias; H Heller; S Ferber; A Hershko
Journal:  J Biol Chem       Date:  1980-08-25       Impact factor: 5.157

7.  Preferential localization of variant nucleosomes near the 5'-end of the mouse dihydrofolate reductase gene.

Authors:  J Barsoum; A Varshavsky
Journal:  J Biol Chem       Date:  1985-06-25       Impact factor: 5.157

8.  Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.

Authors:  L Levinger; A Varshavsky
Journal:  Cell       Date:  1982-02       Impact factor: 41.582

9.  Biosynthesis of the human asialoglycoprotein receptor.

Authors:  A L Schwartz; D Rup
Journal:  J Biol Chem       Date:  1983-09-25       Impact factor: 5.157

10.  Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections.

Authors:  H J Geuze; J W Slot; P A van der Ley; R C Scheffer
Journal:  J Cell Biol       Date:  1981-06       Impact factor: 10.539
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  19 in total

1.  Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.

Authors:  A L Schwartz; J S Trausch; A Ciechanover; J W Slot; H Geuze
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-15       Impact factor: 11.205

2.  Amyloid beta precursor protein and ubiquitin epitopes in human and experimental dystrophic axons. Ultrastructural localization.

Authors:  B Bacci; E Cochran; M G Nunzi; E Izeki; T Mizutani; A Patton; S Hite; L M Sayre; L Autilio-Gambetti; P Gambetti
Journal:  Am J Pathol       Date:  1994-04       Impact factor: 4.307

Review 3.  Autophagic proteolysis: control and specificity.

Authors:  E F Blommaart; J J Luiken; A J Meijer
Journal:  Histochem J       Date:  1997-05

4.  Ubiquitin-protein conjugates accumulate in the lysosomal system of fibroblasts treated with cysteine proteinase inhibitors.

Authors:  F J Doherty; N U Osborn; J A Wassell; P E Heggie; L Laszlo; R J Mayer
Journal:  Biochem J       Date:  1989-10-01       Impact factor: 3.857

Review 5.  The spatial and temporal organization of ubiquitin networks.

Authors:  Caroline Grabbe; Koraljka Husnjak; Ivan Dikic
Journal:  Nat Rev Mol Cell Biol       Date:  2011-03-30       Impact factor: 94.444

6.  Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent.

Authors:  S J Grenfell; J S Trausch-Azar; P M Handley-Gearhart; A Ciechanover; A L Schwartz
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

7.  Synthesis of stress proteins is increased in individuals with homozygous PiZZ alpha 1-antitrypsin deficiency and liver disease.

Authors:  D H Perlmutter; M J Schlesinger; J A Pierce; P I Punsal; A L Schwartz
Journal:  J Clin Invest       Date:  1989-11       Impact factor: 14.808

8.  Ubiquitin-dependent lysosomal degradation of the HNE-modified proteins in lens epithelial cells.

Authors:  Carla Marques; Paulo Pereira; Allen Taylor; Jack N Liang; Venkat N Reddy; Luke I Szweda; Fu Shang
Journal:  FASEB J       Date:  2004-07-09       Impact factor: 5.191

9.  Neurolysosomal pathology in human prosaposin deficiency suggests essential neurotrophic function of prosaposin.

Authors:  Jakub Sikora; Klaus Harzer; Milan Elleder
Journal:  Acta Neuropathol       Date:  2006-10-06       Impact factor: 17.088

10.  Ultrastructural localization of PGP 9.5 and ubiquitin immunoreactivities in rat ductus epididymidis epithelium.

Authors:  R Martín; L Santamaría; B Fraile; R Paniagua; J M Polak
Journal:  Histochem J       Date:  1995-06
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