Characterization of the Indirubin Derivative LDD970 as a Small Molecule Aurora Kinase A Inhibitor in Human Colorectal Cancer Cells.

Aurora kinase A plays an essential role in mitosis including chromosome separation and cytokinesis. Aberrant expression and activity of Aurora kinase A is associated with numerous malignancies including colorectal cancer followed by poor prognosis. The aim of this study is to determine the inhibitory effects of LDD970, an indirubin derivative, on Aurora kinase A in HT29 colorectal cancer cells. In vitro kinase assay revealed that, LDD970 inhibited levels of activated Aurora kinase A (IC50=0.37 mM). The inhibitory effects of LDD970 on Aurora kinase A, autophosphorylation and phosphorylation of histone H3 (Ser10), were confirmed by immunoblot analysis. Moreover, LDD970 inhibited migration of HT29 cells and upregulated apoptosis-related protein cleaved PARP. In cell viability assay, LDD970 was observed to suppress HT29 cell growth (GI50=4.22 µM). Although further studies are required, results of the present study suggest that LDD970 provide a valuable insight into small molecule indirubin derivative for therapeutic potential in human colorectal cancer.

INTRODUCTION

Aurora kinase A belongs to a family of serine/threonine protein kinases that are implicated in important processes during mitosis including centrosome and microtubule activities. Aurora A is involved in controlling several steps of mitosis such as centrosome maturation, bipolar spindle formation (1). Aurora A is phosphorylated on threonine 288 by binding to several protein partners such as targeting protein for Xklp2, p21-activated kinase 1, transforming acidic coiled-coil containing protein 3, and its activity appears to be a peak at G2 phase of the cell cycle (23). Overexpression and amplification of Aurora A has been reported to be detected in a number of solid tumors including colorectal tumors, and aberrant expression of Aurora A induces genomic instability by triggering mitotic checkpoints and apoptosis (4). Moreover, Aurora A overexpression leads to increased degradation of p53, resulting in oncogenic transformation of cells, and contributes to anti-cancer drugs resistance of cells (45). Furthermore, overexpression and amplification of Aurora A is correlated with poor prognosis in patients with colorectal cancers (6). Thus, Aurora A is an attractive target for anticancer therapy due to its essential role in mitotic progression.

It is known that phosphorylation of histone H3 on serine 10 (Ser 10) is required for the proper execution of mitosis (78) and for the initiation of mammalian chromosome condensation (9). In addition, phosphorylation of histone H3 during the G2/M transition is tightly associated with the expression of Aurora A (10).

Several studies have reported that indirubin derivatives inhibit the proliferation of a variety of cells (111213). In the present study, we describe an indirubin derivative LDD970, as a small molecule inhibitor, of Aurora kinase A activity in HT29 colorectal cancer cells.

MATERIALS AND METHODS

Cell culture

HT29 human colorectal cells were purchased from Korean Cell Line Bank (Seoul, Korea) and were cultured in DMEM (Sigma Co., St. Louis, MO, USA) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37℃ in an incubator with an atmosphere of 5% CO2.

In vitro kinase assay

Inhibitory activity of LDD970 against Aurora A kinases was measured by performing HTRF assays. The enzyme was mixed with serially diluted compounds and peptide substrates in a kinase reaction buffer (250 mM HEPES (pH 7.0), 0.1 mM sodium orthovanadate, 5 mM MgCl2, 1 mM DTT, 0.01% bovine serum albumin, 0.02% NaN3). After the addition of detection reagents, TR-FRET signal was measured using Victor multilabel reader (Perkin Elmer, Waltham, MA, USA). IC50 was calculated using nonlinear regression with Prism version 5.01 (GraphPad, La Jolla, CA, USA).

Cell viability assay

Cell viability was measured by performing a tetrazolium-based assay with EZ-Cytox Cell Viability Assay Kit (DaeilLab, Korea). Briefly, HT29 cells were seeded in 96-well plates at a density of 1,000 cells/well and were incubated at 37℃ for 24 h. The cells were treated with serially diluted LDD970 for 72 h. Next, 15 µL EZ-Cytox reagent was added to each well, and the plates were incubated at 37℃ for 4 h. Absorbance was measured using Victor multi-label reader, and IC50 was calculated using nonlinear regression using Prism version 5.01.

Immunoblot analysis

Expression levels and phosphorylation of Aurora A and histone H3 were evaluated by performing immunoblotting analysis as described previously (14). Antibodies against Aurora A (p-Aurora A) and PARP were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-histone H3 antibody and anti-phosphorylated histone H3 (anti-p-H3; Ser10) antibody were obtained from Abcam (Cambridge, MA, USA) and Millipore (Billerica, MA, USA), respectively. The monoclonal antibody against β-actin (Sigma-Aldrich, St. Louis, MO, USA) was used for a loading control.

Wound healing assay

HT29 cells were plated on 6-well plates and were cultured until they reached 90% confluence. A 10 µL tip was used to create a 2 mm wound in the cell monolayer. Detached cells were removed by washing with DMEM. Cells in the plate were treated with LDD970 and were allowed to migrate for 24 and 48 h. Images of live cells were obtained using a phase-contrast microscope (Carl Zeiss, Germany).

RESULTS

Effect of LDD970 on Aurora A kinase activity

LDD970 (Fig. 1A) was tested against Aurora A kinase activity using purified recombinant Aurora A protein. Aurora A inhibition was measured by performing the HTRF assay as described in Materials and Methods. LDD970 exhibited potent inhibitory activity with an IC50 of 0.37 µM. Inhibitory activities of LDD970 against other kinases are listed in Table I. LDD970 did not affected the in vitro kinase activities of c-Met, ALK, and JAK2 (IC50>10 µM). Moreover, LDD970 was likely to show selectivity toward Aurora A among all the kinases tested despite the use of small number of kinases.

Figure 1

Inhibition of Aurora kinase A by LDD970. (A) Chemical structure of LDD970 compound. (B) Effect of LDD970 on the in vitro kinase activity of Aurora A. This assay was performed on purified recombinant Aurora A enzyme using HTRF method.

Table I

Inhibitory activity against select kinases

Kinase	IC50 (µM)
c-Met	>10 µM
ALK	>10 µM
JAK2	>10 µM

The inhibitory activities of LDD970 were evaluated against various purified recombinant kinases using HTRF method.

Inhibition of Aurora A autophosphorylation by LDD970 in HT29 cells

Upon activation, Aurora kinase A undergoes autophosphorylation at threonine 288 (3). Histone H3 is a substrate of Aurora A, and is phosphorylated by Aurora A at Ser10 (7). To determine the inhibitory effects of LDD970 on Aurora A in HT29 cells, we determined p-Aurora A and p-H3 (Ser10) levels in HT29 cells by performing immunoblotting analysis. Our results showed that LDD970 treatment decreased the levels of p-Aurora A and p-H3; however, no change was observed in the total expression level of histone H3 (Fig. 2A). Further, to confirm that LDD970 inhibited Aurora A kinase activity, we performed a time-course experiment to measure p-H3 levels in HT29 cells. Treatment of cells with LDD970 decreased p-H3 levels in a time-dependent manner. Even the inhibitory effect of LDD970 on histone H3 phosphorylation was found to occur before 1 h after treatment of 1 µM and 10 µM of LDD970 (Fig. 2B).

Figure 2

Effects of LDD970 on phosphorylation of Aurora A and histone H3 in HT29 cells. Immunoblot analysis of phosphorylated Aurora A (p-Aurora A), phosphorylated histone H3 (p-H3) on Ser10 on HT29 cells. Cells were treated with LDD970 for 2 h with indicated concentration (A) or indicated times with 1 µM and 10 µM concentration (B). Lysates were analyzed using antibodies against p-Aurora A, p-H3 on Ser10. The β-actin antibody was used as a loading control.

Effects of LDD970 on HT29 cell growth and apoptosis

Cytotoxicity of LDD970 was measured to evaluate HT29 colorectal cancer cells growth. Cells were treated with LDD970 for 72 h, and cell viability was measured. We found that LDD970 treatment suppressed the growth of HT29 cells with IC50 of 4.22 µM (Fig. 3A).

Figure 3

Effects of LDD970 on cell growth and apoptosis. (A) Determination of cytotoxicity activity of LDD970 on HT29 cells. Cells were treated with LDD970 for 72 h. Percentage of cell growth was calculated using 0.5% DMSO treatment as a negative control. (B) Immunoblotting analysis of PARP and cleaved PARP after 48 h of treatment of LDD970.

Inhibition of Aurora A activity induced mitotic spindle defect resulting in cell death through apoptosis (2). LDD970-mediated induction of apoptosis was confirmed by evaluating the levels of PARP cleavage. We observed a dose-dependent increase in PARP cleavage (Fig. 3B), indicating that LDD970 induced the apoptosis of HT29 cells.

Inhibition of HT29 cell migration by LDD970

Cell migration was assessed by performing the wound healing assay, followed by LDD970 treatment. After wound induction, cells were washed and were treated with the indicated concentrations of LDD970. LDD970 treatment suppressed the migration of HT29 cells in a concentration-dependent manner as compared to untreated cells (Fig. 4).

Figure 4

Inhibition of cell migration by LDD970. After making the wound, cells were washed and treated with the indicated concentrations of LDD970. Cells were photographed at the time scratch (Time 0, upper panel). Movement of cells into the wound was observed after 24 h (middle panel) and 48 h (lower panel) using a microscope. Cell migration was assessed by recovery of the scratch.

DISCUSSION

The microtubules play an important role during mitosis (15), providing an ideal drug target for cancer therapy. It has been demonstrated that antimitotic drugs such as taxanes and vinca alkaloids have specific interaction with microtubules. They inhibit the microtubule dynamics by disturbing the spindle assembly checkpoint (16). Although these drugs are currently the best approach for treatment of variety of tumors, there are still issues on severe side effects. Moreover, Antimitotic agents exert cytotoxic effects on non-tumorigenic cells and induce drug resistance (16). Development of anti-cancer agents that disrupt mitosis and enhance therapeutic potency with fewer side effects is the main focus of new Antimitotic drug research. Aurora A is a mitotic specific kinase that has been identified as a promising target for anti-cancer therapy.

In this study, we reported that LDD970, an indirubin derivative, possessed inhibitory activity against Aurora kinase A, resulting in decreased proliferative capacity and viability, and inhibition of migration as well as induction of apoptosis in HT29 human colorectal cancer cells. This result clearly showed that LDD970 displayed a potent inhibitory activity against Aurora kinase A. Inhibition of Aurora kinase A activity using Aurora A recombinant enzyme was attested by the evaluation of autophosphorylation of histone H3 on serine 10. The phosphorylation of histone H3 (Ser10) plays an important role in chromosome condensation and is controlled by Aurora kinase A during G2/M transition (10). Following inhibition of histone H3 phosphorylation, chromosome condensation is prevented and entry to mitosis is knocked down. The levels of histone H3 phosphorylation were measured to determine whether LDD970 had the effect on the cells. Our data revealed that the levels of phosphorylated histone H3 were inhibited with LDD970 treatment in HT29 cells. Moreover, exposure to LDD970 was found to inhibit phosphorylation of histone H3 in both short-term and long-term treated periods. These results were consistent with previous reports that treatment of Aurora A inhibitors in human cancer cells overexpressing Aurora A resulted in loss of phosphorylated histone H3 (17). This indicates that LDD970 is an Aurora A kinase inhibitor since phosphorylation of histone H3 is a direct downstream target of Aurora kinase A. Further, using the wound healing assay, we demonstrated that cells treated with LDD970 for 24 h and 48 h showed a reduced capacity to undergo migration. In addition, when LDD970 was applied to HT29 cells, PARP cleavage was increased (18), suggesting that LDD970 induced apoptosis. These results are in agreement with previous findings that treatment with Aurora kinase A inhibitors not only block cell proliferation and migration, but also induce apoptotic cell death (1719).

Aurora A has received much attention because of its important role during mitosis and association with cancer progression. Drugs that inhibit Aurora kinase A have been discovered and currently pursued in stages of preclinical and clinical development (20). However, further in-depth studies require increasing the efficacy and reducing side effects. This LDD970 study provides useful pharmacological information in the field of Aurora A research.