Literature DB >> 2844162

5'-Nucleotidases in rat heart. Evidence for the occurrence of two soluble enzymes with different substrate specificities.

V L Truong1, A R Collinson, J M Lowenstein.   

Abstract

Chromatography of soluble proteins from rat heart on phosphocellulose columns separates two 5'-nucleotidases. The first to emerge from the column shows a preference for AMP over IMP as substrate, whereas the second shows a preference for IMP over AMP. The properties of the IMP-preferring enzyme, including the conditions under which it is eluted from phosphocellulose columns, show it to be the enzyme studied by Itoh, Oka & Ozasa [Biochem. J. (1986) 235, 847-851]. The kinetic properties of the AMP-preferring enzyme indicate that it is likely to be the enzyme responsible for the production of adenosine under conditions of hypoxia and increased work load, and with metabolic stresses such as a high load of acetate.

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Year:  1988        PMID： 2844162      PMCID： PMC1149265          DOI： 10.1042/bj2530117

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Cytosol 5'-nucleotidase from chicken liver. Purification and some properties.

Authors:  Y Naito; K Tsushima
Journal:  Biochim Biophys Acta       Date:  1976-06-07

3.  Evidence for existence of a cytosol 5'-nucleotidase in chicken heart: comparison of some properties of heart and liver enzymes.

Authors:  R Itoh; J Oka
Journal:  Comp Biochem Physiol B       Date:  1985

4.  Studies on some molecular properties of cytosol 5'-nucleotidase from rat liver.

Authors:  R Itoh
Journal:  Biochim Biophys Acta       Date:  1982-05-05

5.  Adenine nucleotide metabolism and compartmentalization in isolated adult rat heart cells.

Authors:  T Geisbuhler; R A Altschuld; R W Trewyn; A Z Ansel; K Lamka; G P Brierley
Journal:  Circ Res       Date:  1984-05       Impact factor: 17.367

6.  Prolonged myocardial nucleotide depletion after brief ischemia in the open-chest dog.

Authors:  J L Swain; R L Sabina; P A McHale; J C Greenfield; E W Holmes
Journal:  Am J Physiol       Date:  1982-05

7.  Adenosine formation and release from neonatal-rat heart cells in culture.

Authors:  P Meghji; C A Holmquist; A C Newby
Journal:  Biochem J       Date:  1985-08-01       Impact factor: 3.857

8.  Metabolic control of the circulation. Effects of acetate and pyruvate.

Authors:  C S Liang; J M Lowenstein
Journal:  J Clin Invest       Date:  1978-11       Impact factor: 14.808

9.  Different sites of adenosine formation in the heart.

Authors:  W Schütz; J Schrader; E Gerlach
Journal:  Am J Physiol       Date:  1981-06

10.  Purification and some properties of cytosol 5'-nucleotidase from rat liver.

Authors:  R Itoh
Journal:  Biochim Biophys Acta       Date:  1981-02-13
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  20 in total

1.  5'-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol-glycan.

Authors:  U Stochaj; K Flocke; W Mathes; H G Mannherz
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

2.  Inhibition of IMP-specific cytosolic 5'-nucleotidase and adenosine formation in rat polymorphonuclear leucocytes by 5'-deoxy-5'-isobutylthio derivatives of adenosine and inosine.

Authors:  A C Skladanowski; G B Sala; A C Newby
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

3.  Coronary autoregulation and purine release in normoxic heart at various cytoplasmic phosphorylation potentials: disparate effects of adenosine.

Authors:  Y H Kang; R T Mallet; R Bünger
Journal:  Pflugers Arch       Date:  1992-06       Impact factor: 3.657

Review 4.  5'-Nucleotidase: molecular structure and functional aspects.

Authors:  H Zimmermann
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

5.  Nucleotide ectoenzyme activities of human and Chinese hamster fibroblasts in tissue culture.

Authors:  J M Boyle; Y Hey; M Fox
Journal:  Biochem Genet       Date:  1989-12       Impact factor: 1.890

6.  Affinity labelling of 5'-nucleotidases with 5'-p-fluorosulphonylbenzoyladenosine.

Authors:  U Stochaj; H G Mannherz
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

7.  Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.

Authors:  C Fini; V D Thuong; M Aliante; A Floridi; S Cannistraro
Journal:  J Membr Biol       Date:  1994-10       Impact factor: 1.843

8.  Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.

Authors:  P Meghji; A C Skladanowski; A C Newby; L L Slakey; J D Pearson
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

9.  Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.

Authors:  A C Skladanowski; A C Newby
Journal:  Biochem J       Date:  1990-05-15       Impact factor: 3.857

10.  The influence of calcium antagonists on the adenine nucleotide metabolism in the guinea-pig working heart during ischaemia and reperfusion.

Authors:  J G Hugtenburg; M J Mathy; N de Haan; J J Beckeringh; P A van Zwieten
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1991-05       Impact factor: 3.000
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