Kyung-Ku Kang1,2, Eun-Joo Lee1,2, Youg-Deuk Kim1,2, Myung-Jin Chung1,2, Jun-Young Kim3, Shin-Yoon Kim3, Su-Kyeung Hwang4, Kyu-Shik Jeong5,2. 1. Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea. 2. Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea. 3. Department of Orthopedic Surgery, Kyungpook National University Hospital, Daegu, Republic of Korea. 4. Department of Pediatrics, Kyungpook National University Hospital, Daegu, Republic of Korea. 5. Department of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea jeongks@knu.ac.kr.
Abstract
BACKGROUND/AIM: We hypothesized that combined therapy using adipose-derived stem cells (ASCs) and vitamin C might improve tendon regeneration in tendonitis. To determine combined effects of ASC transplantation with vitamin C, we used senescence marker protein 30 (SMP30)-knockout (KO) mice that cannot biosynthesize vitamin C by themselves. MATERIALS AND METHODS: SMP30-KO were divided into four groups: Control, vitamin C, ASCs, and vitamin C plus ASCs. Tendonitis was induced in the achilles tendons via injection of collagenase type I. After 1 week, ASCs were injected into the intratendonal region. After 30 days, all mice were sacrificed and Achilles tendons were isolated. RESULTS: Gross and microscopic findings showed mice treated with combination of ASC transplantation and vitamin C showed better tendon regeneration than those in other groups. This combination led to higher serum vitamin C levels than use of vitamin C alone. This indicates that the vitamin C-treated group used more vitamin C as a precursor to collagen synthesis, whereas vitamin C was in excess in the combination group because of the added effect of ASCs on tendon healing. CONCLUSION: This study showed that vitamin C improved the effect of ASC transplantation on tendonitis by inducing a better stem cell niche. Copyright
BACKGROUND/AIM: We hypothesized that combined therapy using adipose-derived stem cells (ASCs) and vitamin C might improve tendon regeneration in tendonitis. To determine combined effects of ASC transplantation with vitamin C, we used senescence marker protein 30 (SMP30)-knockout (KO) mice that cannot biosynthesize vitamin C by themselves. MATERIALS AND METHODS:SMP30-KO were divided into four groups: Control, vitamin C, ASCs, and vitamin C plus ASCs. Tendonitis was induced in the achilles tendons via injection of collagenase type I. After 1 week, ASCs were injected into the intratendonal region. After 30 days, all mice were sacrificed and Achilles tendons were isolated. RESULTS: Gross and microscopic findings showed mice treated with combination of ASC transplantation and vitamin C showed better tendon regeneration than those in other groups. This combination led to higher serum vitamin C levels than use of vitamin C alone. This indicates that the vitamin C-treated group used more vitamin C as a precursor to collagen synthesis, whereas vitamin C was in excess in the combination group because of the added effect of ASCs on tendon healing. CONCLUSION: This study showed that vitamin C improved the effect of ASC transplantation on tendonitis by inducing a better stem cell niche. Copyright
Authors: Karolina Bochon; Katarzyna Zielniok; Maciej Gawlak; Katarzyna Zawada; Weronika Zarychta-Wiśniewska; Katarzyna Siennicka; Sławomir Struzik; Leszek Pączek; Anna Burdzińska Journal: Int J Stem Cells Date: 2021-02-28 Impact factor: 2.500